TY - JOUR
T1 - Imaging the response to deep brain stimulation in rodent using functional ultrasound
AU - Nayak, Rohit
AU - Lee, Jeyeon
AU - Chantigian, Siobhan
AU - Fatemi, Mostafa
AU - Chang, Su Youne
AU - Alizad, Azra
N1 - Publisher Copyright:
© 2021 Institute of Physics and Engineering in Medicine.
PY - 2021/3/7
Y1 - 2021/3/7
N2 - In this study, we explored the feasibility of using functional ultrasound (fUS) imaging to visualize cerebral activation associated with thalamic deep brain stimulation (DBS), in rodents. The ventrolateral (VL) thalamus was stimulated using electrical pulses of low and high frequencies of 10 and 100 Hz, respectively, and multiple voltages (1-7 V) and pulse widths (50-1500 μs). The fUS imaging demonstrated DBS-evoked activation of cerebral cortex based on changes of cerebral blood volume, specifically at the primary motor cortex (PMC). Low frequency stimulation (LFS) demonstrated significantly higher PMC activation compared to higher frequency stimulation (HFS), at intensities (5-7 V). Whereas, at lower intensities (1-3 V), only HFS demonstrated visible PMC activation. Further, LFS-evoked cerebral activation was was primarily located at the PMC. Our data presents the functionality and feasibility of fUS imaging as an investigational tool to identify brain areas associated with DBS. This preliminary study is an important stepping stone towards conducting real-time functional ultrasound imaging of DBS in awake and behaving animal models, which is of significant interest to the community for studying motor-related disorders.
AB - In this study, we explored the feasibility of using functional ultrasound (fUS) imaging to visualize cerebral activation associated with thalamic deep brain stimulation (DBS), in rodents. The ventrolateral (VL) thalamus was stimulated using electrical pulses of low and high frequencies of 10 and 100 Hz, respectively, and multiple voltages (1-7 V) and pulse widths (50-1500 μs). The fUS imaging demonstrated DBS-evoked activation of cerebral cortex based on changes of cerebral blood volume, specifically at the primary motor cortex (PMC). Low frequency stimulation (LFS) demonstrated significantly higher PMC activation compared to higher frequency stimulation (HFS), at intensities (5-7 V). Whereas, at lower intensities (1-3 V), only HFS demonstrated visible PMC activation. Further, LFS-evoked cerebral activation was was primarily located at the PMC. Our data presents the functionality and feasibility of fUS imaging as an investigational tool to identify brain areas associated with DBS. This preliminary study is an important stepping stone towards conducting real-time functional ultrasound imaging of DBS in awake and behaving animal models, which is of significant interest to the community for studying motor-related disorders.
KW - Cerebral hemodynamics
KW - Deep brain stimulation
KW - Functional ultrasound
KW - Microvasculature imaging
KW - Thalamus stimulation
UR - http://www.scopus.com/inward/record.url?scp=85101828947&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85101828947&partnerID=8YFLogxK
U2 - 10.1088/1361-6560/abdee5
DO - 10.1088/1361-6560/abdee5
M3 - Article
C2 - 33482648
AN - SCOPUS:85101828947
SN - 0031-9155
VL - 66
JO - Physics in medicine and biology
JF - Physics in medicine and biology
IS - 5
M1 - 05LT01
ER -