Image thresholding is a challenging task in image processing field. Many efforts have already been made to propose universal, robust methods to handle a wide range of images. Previously by the same authors, an optimization-based thresholding approach was introduced. According to the proposed approach, Differential Evolution (DE) algorithm, minimizes dissimilarity between the input grey-level image and the bi-level (thresholded) image. In the current paper, micro Opposition-Based Differential Evolution (micro-ODE), DE with very small population size and opposition-based population initialization, has been proposed. Then, it is compared with a well-known thresholding method, Kittler algorithm and also with its non-opposition-based version (micro-DE). In overall, the proposed approach outperforms Kittler method over 16 challenging test images. Furthermore, the results confirm that the micro-ODE is faster than micro-DE because of embedding the opposition-based population initialization.