IL-10 induces the development of immunosuppressive CD14+ HLA-DRlow/- monocytes in B-cell non-Hodgkin lymphoma

B. Xiu, Y. Lin, D. M. Grote, S. C. Ziesmer, M. P. Gustafson, M. L. Maas, Z. Zhang, A. B. Dietz, L. F. Porrata, A. J. Novak, A. B. Liang, Z. Z. Yang, S. M. Ansell

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The biological role of monocytes and macrophages in B-cell non-Hodgkin lymphoma (NHL) is not fully understood. We have previously reported that monocytes from patients with B-cell NHL have an immunosuppressive CD14+HLA-DRlow/- phenotype that correlates with a poor prognosis. However, the underlying mechanism by which CD14+HLA-DRlow/- monocytes develop in lymphoma is unknown. In the present study, we found that interleukin (IL)-10, which is increased in the serum of patients with B-cell NHL, induced the development of the CD4+HLA-DRlow/- population. Using peripheral blood samples from patients with B-cell NHL, we found that absolute numbers of CD14+ monocytic cells with an HLA-DRlow/- phenotype were higher than healthy controls and correlated with a higher International Prognostic Index score. IL-10 serum levels were elevated in lymphoma patients compared with controls and were associated with increased peripheral monocyte counts. Treatment of monocytes with IL-10 in vitro significantly decreased HLA-DR expression and resulted in the expansion of CD14+HLA-DRlow/- population. We found that lymphoma B cells produce IL-10 and supernatants from cultured lymphoma cells increased the CD14+HLA-DRlow/- population. Furthermore, we found that IL-10-induced CD14+HLA-DRlow/- monocytes inhibited the activation and proliferation of T cells. Taken together, these results suggest that elevated IL-10 serum levels contribute to increased numbers of immunosuppressive CD14+HLADRlow/- monocytes in B-cell NHL.

Original languageEnglish (US)
Article numbere328
JournalBlood cancer journal
Volume5
Issue number7
DOIs
StatePublished - Jul 31 2015

ASJC Scopus subject areas

  • Hematology
  • Oncology

Fingerprint Dive into the research topics of 'IL-10 induces the development of immunosuppressive CD14<sup>+</sup> HLA-DR<sup>low/-</sup> monocytes in B-cell non-Hodgkin lymphoma'. Together they form a unique fingerprint.

  • Cite this