@article{e526b5b79ef44f3b9309c5e5a0d52cd9,
title = "IFN-γ gene expression is controlled by the architectural transcription factor HMGA1",
abstract = "We report for the first time that IFNG gene expression requires high mobility group (HMG)A1, the architectural transcription factor mediating enhanceosome formation. This finding is supported by our direct studies of T cells isolated from the HMGA1-transgenic mice displaying an up-regulation of IFN-γ production and of HMGA1-deficient mice exhibited a decreased IFN-γ induction. In parallel transfection studies in EL4 cells, we observed elevated IFNG gene promoter activity in cells stably over-expressing HMGA1 and a reduction of such activity in cells expressing dominant-negative HMGA1. In vitro binding assays further demonstrated a specific interaction of HMGA1 to defined regions of the IFNG gene proximal promoter.",
keywords = "Gene regulation, HMGA1, IFN-γ",
author = "Chau, {Kai Yin} and Keane-Myers, {Andrea M.} and Monica Fedele and Yasuhiro Ikeda and Creusot, {Remi J.} and Lisa Menozzi and Cousins, {David J.} and Guidalberto Manfioletti and Lionel Feigenbaum and Alfredo Fusco and Ono, {Santa Jeremy}",
note = "Funding Information: This study was supported in part by the National Institutes of Health, USA (R01 GM49661), the Lucille P. Markey Foundation and the Wellcome Trust to S.J.O. The generous support of Fight for Sight and GlaxoSmithKline is also acknowledged. We thank Thomas M. Aune for providing the panel of human IFNG promoter reporter constructs, Raymond Reeves for providing the plasmid expressing the dominant-negative mutant of HMGA1, Mary Collins for sharing the recombinant lentiviral gene transfer technology and Roger M. Perlmutter for providing plasmid pW120. We are in debt to Tasuku Honjo for his generous help in this work.",
year = "2005",
month = mar,
doi = "10.1093/intimm/dxh209",
language = "English (US)",
volume = "17",
pages = "297--306",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "3",
}