IFN-γ gene expression is controlled by the architectural transcription factor HMGA1

Kai Yin Chau, Andrea M. Keane-Myers, Monica Fedele, Yasuhiro Ikeda, Remi J. Creusot, Lisa Menozzi, David J. Cousins, Guidalberto Manfioletti, Lionel Feigenbaum, Alfredo Fusco, Santa Jeremy Ono

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We report for the first time that IFNG gene expression requires high mobility group (HMG)A1, the architectural transcription factor mediating enhanceosome formation. This finding is supported by our direct studies of T cells isolated from the HMGA1-transgenic mice displaying an up-regulation of IFN-γ production and of HMGA1-deficient mice exhibited a decreased IFN-γ induction. In parallel transfection studies in EL4 cells, we observed elevated IFNG gene promoter activity in cells stably over-expressing HMGA1 and a reduction of such activity in cells expressing dominant-negative HMGA1. In vitro binding assays further demonstrated a specific interaction of HMGA1 to defined regions of the IFNG gene proximal promoter.

Original languageEnglish (US)
Pages (from-to)297-306
Number of pages10
JournalInternational Immunology
Volume17
Issue number3
DOIs
StatePublished - Mar 1 2005

Keywords

  • Gene regulation
  • HMGA1
  • IFN-γ

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Chau, K. Y., Keane-Myers, A. M., Fedele, M., Ikeda, Y., Creusot, R. J., Menozzi, L., Cousins, D. J., Manfioletti, G., Feigenbaum, L., Fusco, A., & Ono, S. J. (2005). IFN-γ gene expression is controlled by the architectural transcription factor HMGA1. International Immunology, 17(3), 297-306. https://doi.org/10.1093/intimm/dxh209