Identification of targets of miR-200b by a SILAC-based quantitative proteomic approach

Arivusudar Marimuthu, Tai Chung Huang, Lakshmi Dhevi N. Selvan, Santosh Renuse, Raja Sekhar Nirujogi, Praveen Kumar, Sneha M. Pinto, Sudha Rajagopalan, Akhilesh Pandey, H. C. Harsha, Aditi Chatterjee

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

miRNAs regulate gene expression by binding to cognate mRNAs causing mRNA degradation or translational repression. Mass spectrometry-based proteomic analysis is being widely used to identify miRNA targets. The miR-200b miRNA cluster is often overexpressed in multiple cancer types, but the identity of the targets remains elusive. Using SILAC-based analysis, we examined the effects of overexpression of a miR-200b mimic or a control miRNA in fibrosarcoma cells. We identified around 300 potential targets of miR-200b based on a change in the expression of protein levels. We validated a subset of potential targets at the transcript level using quantitative PCR.

Original languageEnglish (US)
Pages (from-to)10-17
Number of pages8
JournalEuPA Open Proteomics
Volume4
DOIs
StatePublished - May 2 2014

Keywords

  • HT-1080
  • Mass spectrometry
  • MiRNA targets

ASJC Scopus subject areas

  • Biochemistry

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    Marimuthu, A., Huang, T. C., Selvan, L. D. N., Renuse, S., Nirujogi, R. S., Kumar, P., Pinto, S. M., Rajagopalan, S., Pandey, A., Harsha, H. C., & Chatterjee, A. (2014). Identification of targets of miR-200b by a SILAC-based quantitative proteomic approach. EuPA Open Proteomics, 4, 10-17. https://doi.org/10.1016/j.euprot.2014.04.006