Abstract
miRNAs regulate gene expression by binding to cognate mRNAs causing mRNA degradation or translational repression. Mass spectrometry-based proteomic analysis is being widely used to identify miRNA targets. The miR-200b miRNA cluster is often overexpressed in multiple cancer types, but the identity of the targets remains elusive. Using SILAC-based analysis, we examined the effects of overexpression of a miR-200b mimic or a control miRNA in fibrosarcoma cells. We identified around 300 potential targets of miR-200b based on a change in the expression of protein levels. We validated a subset of potential targets at the transcript level using quantitative PCR.
Original language | English (US) |
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Pages (from-to) | 10-17 |
Number of pages | 8 |
Journal | EuPA Open Proteomics |
Volume | 4 |
DOIs | |
State | Published - May 2 2014 |
Keywords
- HT-1080
- Mass spectrometry
- MiRNA targets
ASJC Scopus subject areas
- Biochemistry