TY - JOUR
T1 - Identification of protein kinase C and its multiple isoforms in FRTL-5 thyroid cells
AU - Wang, X. D.
AU - Kiang, J. G.
AU - Smallridge, R. C.
PY - 1995
Y1 - 1995
N2 - Protein kinase C (PKC) has been implicated as an important regulator of signal transduction in the FRTL-5 thyroid cell line, but little is known about its isoforms in this cell line. In the present investigation, we characterized the activation of PKC by measuring the enzyme activity and identifying its isoforms in both cytosol and membrane fractions. Phorbol 12- myristate 13-acetate (PMA) was used as a PKC activator in this study. PKC activity assay revealed that PMA (300 nM) induced a rapid translocation from cytosol to membrane within 1 min and led to an almost complete translocation within 15 min. Multiple PKC isoforms were examined by Western blot analysis with specific antibodies against α, β, γ, δ, ε, and ζ isoforms. PKC α, δ, ε, and ζ were identified in this cell line, but PKC β and γ were not. Exposure of the cells to PMA (300 nM) for 5 to 30 min led to the translocation of PKC α, δ, and ε from the cytosol to the membrane fraction, while PKC ζ was not affected. Treatment with PMA (300 nM) for 24 h resulted in the down-regulation of PKC α, δ, and ε, but not PKC ζ. This study demonstrates for the first time direct evidence for the activation of PKC, and expression and distribution of its isoforms in FRTL-5 thyroid cells.
AB - Protein kinase C (PKC) has been implicated as an important regulator of signal transduction in the FRTL-5 thyroid cell line, but little is known about its isoforms in this cell line. In the present investigation, we characterized the activation of PKC by measuring the enzyme activity and identifying its isoforms in both cytosol and membrane fractions. Phorbol 12- myristate 13-acetate (PMA) was used as a PKC activator in this study. PKC activity assay revealed that PMA (300 nM) induced a rapid translocation from cytosol to membrane within 1 min and led to an almost complete translocation within 15 min. Multiple PKC isoforms were examined by Western blot analysis with specific antibodies against α, β, γ, δ, ε, and ζ isoforms. PKC α, δ, ε, and ζ were identified in this cell line, but PKC β and γ were not. Exposure of the cells to PMA (300 nM) for 5 to 30 min led to the translocation of PKC α, δ, and ε from the cytosol to the membrane fraction, while PKC ζ was not affected. Treatment with PMA (300 nM) for 24 h resulted in the down-regulation of PKC α, δ, and ε, but not PKC ζ. This study demonstrates for the first time direct evidence for the activation of PKC, and expression and distribution of its isoforms in FRTL-5 thyroid cells.
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U2 - 10.1089/thy.1995.5.137
DO - 10.1089/thy.1995.5.137
M3 - Article
C2 - 7647574
AN - SCOPUS:0029023303
SN - 1050-7256
VL - 5
SP - 137
EP - 140
JO - Thyroid
JF - Thyroid
IS - 2
ER -