Identification of protein kinase C and its multiple isoforms in FRTL-5 thyroid cells

X. D. Wang, J. G. Kiang, Robert Christian Smallridge

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Protein kinase C (PKC) has been implicated as an important regulator of signal transduction in the FRTL-5 thyroid cell line, but little is known about its isoforms in this cell line. In the present investigation, we characterized the activation of PKC by measuring the enzyme activity and identifying its isoforms in both cytosol and membrane fractions. Phorbol 12- myristate 13-acetate (PMA) was used as a PKC activator in this study. PKC activity assay revealed that PMA (300 nM) induced a rapid translocation from cytosol to membrane within 1 min and led to an almost complete translocation within 15 min. Multiple PKC isoforms were examined by Western blot analysis with specific antibodies against α, β, γ, δ, ε, and ζ isoforms. PKC α, δ, ε, and ζ were identified in this cell line, but PKC β and γ were not. Exposure of the cells to PMA (300 nM) for 5 to 30 min led to the translocation of PKC α, δ, and ε from the cytosol to the membrane fraction, while PKC ζ was not affected. Treatment with PMA (300 nM) for 24 h resulted in the down-regulation of PKC α, δ, and ε, but not PKC ζ. This study demonstrates for the first time direct evidence for the activation of PKC, and expression and distribution of its isoforms in FRTL-5 thyroid cells.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalThyroid
Volume5
Issue number2
StatePublished - 1995
Externally publishedYes

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Protein Kinase C
Thyroid Gland
Protein Isoforms
Acetates
Cytosol
Cell Line
Membranes
Signal Transduction
Down-Regulation
Western Blotting
phorbol-12-myristate
Antibodies

ASJC Scopus subject areas

  • Endocrinology

Cite this

Identification of protein kinase C and its multiple isoforms in FRTL-5 thyroid cells. / Wang, X. D.; Kiang, J. G.; Smallridge, Robert Christian.

In: Thyroid, Vol. 5, No. 2, 1995, p. 137-140.

Research output: Contribution to journalArticle

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