Identification of genes associated with tumorigenesis of retinoblastoma by microarray analysis

Sanjukta Chakraborty, Sonal Khare, Syril Kumar Dorairaj, Venkatesh C. Prabhakaran, D. Ravi Prakash, Arun Kumar

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

There is no report on the gene expression profile of retinoblastoma (Rb). We analyzed the gene expression profile of Rb by the microarray technique. One thousand four genes were upregulated and 481 genes were downregulated. Microarray data were confirmed by semiquantitative RT-PCR for 5 genes in Rb samples: CDC25A, C17orf75, ERBB3, LATS2, and CHFR. Clusters of differentially expressed genes were identified on chromosomes 1, 16, and 17. Based on the expression profile, we hypothesized that the PI3K/AKT/mTOR (insulin signaling) pathway might be dysregulated in Rb. Our semiquantitative RT-PCR analysis of the PIK3CA, AKT1, FRAP1, and RPS6KB1 genes in Rb samples supported this hypothesis. We suggest that known inhibitors of this pathway could be evaluated for the treatment of Rb.

Original languageEnglish (US)
Pages (from-to)344-353
Number of pages10
JournalGenomics
Volume90
Issue number3
DOIs
StatePublished - Sep 2007

Keywords

  • Expression profile
  • Insulin signaling pathway
  • Microarrays
  • Retinoblastoma

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Identification of genes associated with tumorigenesis of retinoblastoma by microarray analysis'. Together they form a unique fingerprint.

  • Cite this