TY - JOUR
T1 - Identification of genes associated with tumorigenesis of retinoblastoma by microarray analysis
AU - Chakraborty, Sanjukta
AU - Khare, Sonal
AU - Dorairaj, Syril Kumar
AU - Prabhakaran, Venkatesh C.
AU - Prakash, D. Ravi
AU - Kumar, Arun
N1 - Funding Information:
We thank the patients, the normal individuals, and their family members for taking part in this study. We also thank Mr. Aiyaz Mohamed and Mr. V. Madavan for technical help. This work was financially supported by a grant from the ICMR, New Delhi. We also thank three anonymous reviewers and the Associate Editor, Professor Sandrine Dudoit, for their valuable suggestions to improve the manuscript.
PY - 2007/9
Y1 - 2007/9
N2 - There is no report on the gene expression profile of retinoblastoma (Rb). We analyzed the gene expression profile of Rb by the microarray technique. One thousand four genes were upregulated and 481 genes were downregulated. Microarray data were confirmed by semiquantitative RT-PCR for 5 genes in Rb samples: CDC25A, C17orf75, ERBB3, LATS2, and CHFR. Clusters of differentially expressed genes were identified on chromosomes 1, 16, and 17. Based on the expression profile, we hypothesized that the PI3K/AKT/mTOR (insulin signaling) pathway might be dysregulated in Rb. Our semiquantitative RT-PCR analysis of the PIK3CA, AKT1, FRAP1, and RPS6KB1 genes in Rb samples supported this hypothesis. We suggest that known inhibitors of this pathway could be evaluated for the treatment of Rb.
AB - There is no report on the gene expression profile of retinoblastoma (Rb). We analyzed the gene expression profile of Rb by the microarray technique. One thousand four genes were upregulated and 481 genes were downregulated. Microarray data were confirmed by semiquantitative RT-PCR for 5 genes in Rb samples: CDC25A, C17orf75, ERBB3, LATS2, and CHFR. Clusters of differentially expressed genes were identified on chromosomes 1, 16, and 17. Based on the expression profile, we hypothesized that the PI3K/AKT/mTOR (insulin signaling) pathway might be dysregulated in Rb. Our semiquantitative RT-PCR analysis of the PIK3CA, AKT1, FRAP1, and RPS6KB1 genes in Rb samples supported this hypothesis. We suggest that known inhibitors of this pathway could be evaluated for the treatment of Rb.
KW - Expression profile
KW - Insulin signaling pathway
KW - Microarrays
KW - Retinoblastoma
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U2 - 10.1016/j.ygeno.2007.05.002
DO - 10.1016/j.ygeno.2007.05.002
M3 - Article
C2 - 17604597
AN - SCOPUS:34547457538
SN - 0888-7543
VL - 90
SP - 344
EP - 353
JO - Genomics
JF - Genomics
IS - 3
ER -