Identification of Drosophila yin and PEPT2 as evolutionarily conserved phagosome-associated muramyl dipeptide transporters

Guillaume M. Charrière, Wai Kee Eddie Ip, Stéphanie Dejardin, Laurent Boyer, Anna Sokolovska, Michael P. Cappillino, Bobby J. Cherayil, Daniel K. Podolsky, Koichi S. Kobayashi, Neal Silverman, Adam Lacy-Hulbert, Lynda M. Stuart

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

NOD2 (nucleotide-binding oligomerization domain containing 2) is an important cytosolic pattern recognition receptor that activates NF-κB and other immune effector pathways such as autophagy and antigen presentation. Despite its intracellular localization, NOD2 participates in sensing of extracellular microbes such as Staphylococcus aureus. NOD2 ligands similar to the minimal synthetic ligand muramyl dipeptide (MDP) are generated by internalization and processing of bacteria in hydrolytic phagolysosomes. However,howthese derived ligands exit this organelle and access the cytosol to activate NOD2 is poorly understood. Here, we address how phagosome-derived NOD2 ligands access the cytosol in human phagocytes. Drawing on data from Drosophila phagosomes, we identify an evolutionarily conserved role of SLC15A transporters, Drosophila Yin and PEPT2, as MDP transporters in fly and human phagocytes, respectively. We show that PEPT2 is highly expressed by human myeloid cells. Ectopic expression of both Yin and PEPT2 increases the sensitivity of NOD2-dependent NF-κB activation. Additionally, we show that PEPT2 associates with phagosome membranes. Together, these data identify Drosophila Yin and PEPT2 as evolutionarily conserved phagosome-associated transporters that are likely to be of particular importance in delivery of bacteria-derived ligands generated in phagosomes to cytosolic sensors recruited to the vicinity of these organelles.

Original languageEnglish (US)
Pages (from-to)20147-20154
Number of pages8
JournalJournal of Biological Chemistry
Volume285
Issue number26
DOIs
StatePublished - Jun 25 2010
Externally publishedYes

Fingerprint

Acetylmuramyl-Alanyl-Isoglutamine
Phagosomes
Oligomerization
Drosophila
Nucleotides
Ligands
Phagocytes
Organelles
Cytosol
Bacteria
Pattern Recognition Receptors
Autophagy
Antigen Presentation
Myeloid Cells
Diptera
Staphylococcus aureus
Chemical activation
Membranes
Antigens
Sensors

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of Drosophila yin and PEPT2 as evolutionarily conserved phagosome-associated muramyl dipeptide transporters. / Charrière, Guillaume M.; Ip, Wai Kee Eddie; Dejardin, Stéphanie; Boyer, Laurent; Sokolovska, Anna; Cappillino, Michael P.; Cherayil, Bobby J.; Podolsky, Daniel K.; Kobayashi, Koichi S.; Silverman, Neal; Lacy-Hulbert, Adam; Stuart, Lynda M.

In: Journal of Biological Chemistry, Vol. 285, No. 26, 25.06.2010, p. 20147-20154.

Research output: Contribution to journalArticle

Charrière, GM, Ip, WKE, Dejardin, S, Boyer, L, Sokolovska, A, Cappillino, MP, Cherayil, BJ, Podolsky, DK, Kobayashi, KS, Silverman, N, Lacy-Hulbert, A & Stuart, LM 2010, 'Identification of Drosophila yin and PEPT2 as evolutionarily conserved phagosome-associated muramyl dipeptide transporters', Journal of Biological Chemistry, vol. 285, no. 26, pp. 20147-20154. https://doi.org/10.1074/jbc.M110.115584
Charrière, Guillaume M. ; Ip, Wai Kee Eddie ; Dejardin, Stéphanie ; Boyer, Laurent ; Sokolovska, Anna ; Cappillino, Michael P. ; Cherayil, Bobby J. ; Podolsky, Daniel K. ; Kobayashi, Koichi S. ; Silverman, Neal ; Lacy-Hulbert, Adam ; Stuart, Lynda M. / Identification of Drosophila yin and PEPT2 as evolutionarily conserved phagosome-associated muramyl dipeptide transporters. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 26. pp. 20147-20154.
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