Identification and chromosomal location of a new tandemly repeated DNA in maize

Chunxian X. Chen; Huihuang H. Yan; Wenxue X. Zhai; Lihuang H. Zhu; Jingsan S. Sun

doi:10.1139/g99-056

Identification and chromosomal location of a new tandemly repeated DNA in maize

Chunxian X. Chen, Huihuang H. Yan, Wenxue X. Zhai, Lihuang H. Zhu, Jingsan S. Sun

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6.

Original language	English (US)
Pages (from-to)	181-184
Number of pages	4
Journal	Genome
Volume	43
Issue number	1
DOIs	https://doi.org/10.1139/g99-056
State	Published - 2000

Keywords

Fluorescent in situ hybridization (FISH)
Satellite DNA
Tandemly repeated DNA
Zea mays

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Genetics

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10.1139/g99-056

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title = "Identification and chromosomal location of a new tandemly repeated DNA in maize",

abstract = "Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6.",

keywords = "Fluorescent in situ hybridization (FISH), Satellite DNA, Tandemly repeated DNA, Zea mays",

author = "Chen, {Chunxian X.} and Yan, {Huihuang H.} and Zhai, {Wenxue X.} and Zhu, {Lihuang H.} and Sun, {Jingsan S.}",

year = "2000",

doi = "10.1139/g99-056",

language = "English (US)",

volume = "43",

pages = "181--184",

journal = "Genome",

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T1 - Identification and chromosomal location of a new tandemly repeated DNA in maize

AU - Chen, Chunxian X.

AU - Yan, Huihuang H.

AU - Zhai, Wenxue X.

AU - Zhu, Lihuang H.

AU - Sun, Jingsan S.

PY - 2000

Y1 - 2000

N2 - Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6.

AB - Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6.

KW - Fluorescent in situ hybridization (FISH)

KW - Satellite DNA

KW - Tandemly repeated DNA

KW - Zea mays

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DO - 10.1139/g99-056

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SP - 181

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JO - Genome

JF - Genome

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ER -

Identification and chromosomal location of a new tandemly repeated DNA in maize

Abstract

Keywords

ASJC Scopus subject areas

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