Identification and chromosomal location of a new tandemly repeated DNA in maize

Chunxian X. Chen, Huihuang D Yan, Wenxue X. Zhai, Lihuang H. Zhu, Jingsan S. Sun

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6.

Original languageEnglish (US)
Pages (from-to)181-184
Number of pages4
JournalGenome
Volume43
Issue number1
StatePublished - 2000
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 6
Zea mays
chromosomes
Chromosomes, Human, Pair 3
corn
Genomic Library
DNA libraries
new family
DNA
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
Gene Library
methylation
Methylation
Clone Cells
Genome
clones
genomics
nucleotide sequences
genome

Keywords

  • Fluorescent in situ hybridization (FISH)
  • Satellite DNA
  • Tandemly repeated DNA
  • Zea mays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

Chen, C. X., Yan, H. D., Zhai, W. X., Zhu, L. H., & Sun, J. S. (2000). Identification and chromosomal location of a new tandemly repeated DNA in maize. Genome, 43(1), 181-184.

Identification and chromosomal location of a new tandemly repeated DNA in maize. / Chen, Chunxian X.; Yan, Huihuang D; Zhai, Wenxue X.; Zhu, Lihuang H.; Sun, Jingsan S.

In: Genome, Vol. 43, No. 1, 2000, p. 181-184.

Research output: Contribution to journalArticle

Chen, CX, Yan, HD, Zhai, WX, Zhu, LH & Sun, JS 2000, 'Identification and chromosomal location of a new tandemly repeated DNA in maize', Genome, vol. 43, no. 1, pp. 181-184.
Chen, Chunxian X. ; Yan, Huihuang D ; Zhai, Wenxue X. ; Zhu, Lihuang H. ; Sun, Jingsan S. / Identification and chromosomal location of a new tandemly repeated DNA in maize. In: Genome. 2000 ; Vol. 43, No. 1. pp. 181-184.
@article{6151608771b541348d8371fbbb283810,
title = "Identification and chromosomal location of a new tandemly repeated DNA in maize",
abstract = "Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6.",
keywords = "Fluorescent in situ hybridization (FISH), Satellite DNA, Tandemly repeated DNA, Zea mays",
author = "Chen, {Chunxian X.} and Yan, {Huihuang D} and Zhai, {Wenxue X.} and Zhu, {Lihuang H.} and Sun, {Jingsan S.}",
year = "2000",
language = "English (US)",
volume = "43",
pages = "181--184",
journal = "Genome / National Research Council Canada = Genome / Conseil national de recherches Canada",
issn = "0831-2796",
publisher = "National Research Council of Canada",
number = "1",

}

TY - JOUR

T1 - Identification and chromosomal location of a new tandemly repeated DNA in maize

AU - Chen, Chunxian X.

AU - Yan, Huihuang D

AU - Zhai, Wenxue X.

AU - Zhu, Lihuang H.

AU - Sun, Jingsan S.

PY - 2000

Y1 - 2000

N2 - Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6.

AB - Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6.

KW - Fluorescent in situ hybridization (FISH)

KW - Satellite DNA

KW - Tandemly repeated DNA

KW - Zea mays

UR - http://www.scopus.com/inward/record.url?scp=0034102072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034102072&partnerID=8YFLogxK

M3 - Article

C2 - 10701128

AN - SCOPUS:0034102072

VL - 43

SP - 181

EP - 184

JO - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada

JF - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada

SN - 0831-2796

IS - 1

ER -