Hypothalamic distribution of somatostatin mRNA expressing neurones relative to pubertal and adult changes in pulsatile growth hormone secretion in mice

H. Y. Tan, L. Huang, D. Simmons, Johannes D Veldhuis, F. J. Steyn, C. Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The age-associated decline in growth hormone (GH) secretion may be a consequence of the reduction in the number of GH-releasing hormone (GHRH) positive neurones. However, it remains unclear whether an alteration in the number or distribution of somatostatin (SST) neurones contributes to this change. In the present study, we characterised the role of SST in modulating the change in pulsatile GH secretion in male C57Bl/6J mice throughout puberty and into early adulthood. We assessed pulsatile GH secretion in mice at 4, 8 and 16 weeks of age. These ages correspond to early pubertal, early adulthood and adulthood, respectively. We show an elevation in peak, total and pulsatile GH secretion coinciding with periods of rapid linear growth. Using in situ hybridisation and morphometric methods, we mapped the distribution of Sst mRNA expression within the mouse brain relative to this change in pulsatile GH secretion. The results obtained show that altered pulsatile GH secretion in male mice from 4-16 weeks of age does not coincide with a significant change in the number of Sst mRNA expressing neurones or an abundance of Sst mRNA expression throughout the arcuate nucleus (ARC) and periventricular nucleus (PeV). Rather, we observed a progressive decline in Sst mRNA expressing neurones within subnuclei of the paraventricular nucleus at this time. We conclude that structural changes in Sst expression within the PeV and ARC may not reflect the observed decline in pulsatile GH secretion in mice from puberty into early adulthood.

Original languageEnglish (US)
Pages (from-to)910-919
Number of pages10
JournalJournal of Neuroendocrinology
Volume25
Issue number10
DOIs
StatePublished - Oct 2013

Fingerprint

Somatostatin
Growth Hormone
Neurons
Messenger RNA
Arcuate Nucleus of Hypothalamus
Puberty
Growth Hormone-Releasing Hormone
Paraventricular Hypothalamic Nucleus
In Situ Hybridization
Brain
Growth

Keywords

  • Growth hormone
  • Hypothalamus
  • Mouse
  • Pubertal
  • Somatostatin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Cite this

Hypothalamic distribution of somatostatin mRNA expressing neurones relative to pubertal and adult changes in pulsatile growth hormone secretion in mice. / Tan, H. Y.; Huang, L.; Simmons, D.; Veldhuis, Johannes D; Steyn, F. J.; Chen, C.

In: Journal of Neuroendocrinology, Vol. 25, No. 10, 10.2013, p. 910-919.

Research output: Contribution to journalArticle

Tan, H. Y. ; Huang, L. ; Simmons, D. ; Veldhuis, Johannes D ; Steyn, F. J. ; Chen, C. / Hypothalamic distribution of somatostatin mRNA expressing neurones relative to pubertal and adult changes in pulsatile growth hormone secretion in mice. In: Journal of Neuroendocrinology. 2013 ; Vol. 25, No. 10. pp. 910-919.
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