Human RPE like cell culture on biodegradable polymer substrates

Lichun Lu, Robert C. Thomson, Antonios G. Mikos, Richard C. Hunt, Giovanni G. Giordano, Charles A. Garcia, Devjani Lahiri-Munir

Research output: Contribution to journalArticle

Abstract

Purpose. To evaluate the morphology, structure and differentiated function of cultured human D407 retinal pigment epithelium (RPE) like cells on synthetic biodegradable polymer substrates. Methods, Biodegradable polymer films of 50:50 poly(DL-lactic-co-glycolic acid) (PLGA) and 75:25 PLGA were manufactured using a solvent casting technique. Human D407 RPE like cells were cultured on these films at a seeding density of 107,000 cells/cm2. The morphological and structural properties of the cells were investigated by light and electron microscopy. Results, Human D407 RPE like cells attached and proliferated on all the polymer substrates tested, comparable to the control tissue culture polystyrene. For example, 68.1% of the cells plated attached after 8 hrs to 75:25 PLGA films. After 4 days in culture, the cells reached confluency with a density of 1,062,000 cells/cm2 and possessed normal epithelial cobblestone morphology. Conclusions. Biodegradable polymer films can provide suitable substrates for human RPE like cell culture, and hold promise as temporary carriers for subretinal implantation of organized sheets of RPE cells.

Original languageEnglish (US)
Pages (from-to)S98
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Lu, L., Thomson, R. C., Mikos, A. G., Hunt, R. C., Giordano, G. G., Garcia, C. A., & Lahiri-Munir, D. (1996). Human RPE like cell culture on biodegradable polymer substrates. Investigative Ophthalmology and Visual Science, 37(3), S98.