Human RPE like cell culture on biodegradable polymer substrates

Lichun Lu, Robert C. Thomson, Antonios G. Mikos, Richard C. Hunt, Giovanni G. Giordano, Charles A. Garcia, Devjani Lahiri-Munir

Research output: Contribution to journalArticle

Abstract

Purpose. To evaluate the morphology, structure and differentiated function of cultured human D407 retinal pigment epithelium (RPE) like cells on synthetic biodegradable polymer substrates. Methods, Biodegradable polymer films of 50:50 poly(DL-lactic-co-glycolic acid) (PLGA) and 75:25 PLGA were manufactured using a solvent casting technique. Human D407 RPE like cells were cultured on these films at a seeding density of 107,000 cells/cm2. The morphological and structural properties of the cells were investigated by light and electron microscopy. Results, Human D407 RPE like cells attached and proliferated on all the polymer substrates tested, comparable to the control tissue culture polystyrene. For example, 68.1% of the cells plated attached after 8 hrs to 75:25 PLGA films. After 4 days in culture, the cells reached confluency with a density of 1,062,000 cells/cm2 and possessed normal epithelial cobblestone morphology. Conclusions. Biodegradable polymer films can provide suitable substrates for human RPE like cell culture, and hold promise as temporary carriers for subretinal implantation of organized sheets of RPE cells.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

Fingerprint

Retinal Pigment Epithelium
Polymers
Cell Culture Techniques
Artificial Cells
Polystyrenes
Cultured Cells
Electron Microscopy
Light
polylactic acid-polyglycolic acid copolymer

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Lu, L., Thomson, R. C., Mikos, A. G., Hunt, R. C., Giordano, G. G., Garcia, C. A., & Lahiri-Munir, D. (1996). Human RPE like cell culture on biodegradable polymer substrates. Investigative Ophthalmology and Visual Science, 37(3).

Human RPE like cell culture on biodegradable polymer substrates. / Lu, Lichun; Thomson, Robert C.; Mikos, Antonios G.; Hunt, Richard C.; Giordano, Giovanni G.; Garcia, Charles A.; Lahiri-Munir, Devjani.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Lu, L, Thomson, RC, Mikos, AG, Hunt, RC, Giordano, GG, Garcia, CA & Lahiri-Munir, D 1996, 'Human RPE like cell culture on biodegradable polymer substrates', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Lu L, Thomson RC, Mikos AG, Hunt RC, Giordano GG, Garcia CA et al. Human RPE like cell culture on biodegradable polymer substrates. Investigative Ophthalmology and Visual Science. 1996 Feb 15;37(3).
Lu, Lichun ; Thomson, Robert C. ; Mikos, Antonios G. ; Hunt, Richard C. ; Giordano, Giovanni G. ; Garcia, Charles A. ; Lahiri-Munir, Devjani. / Human RPE like cell culture on biodegradable polymer substrates. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
@article{ea4243eca5804c90835e499f1f1541ce,
title = "Human RPE like cell culture on biodegradable polymer substrates",
abstract = "Purpose. To evaluate the morphology, structure and differentiated function of cultured human D407 retinal pigment epithelium (RPE) like cells on synthetic biodegradable polymer substrates. Methods, Biodegradable polymer films of 50:50 poly(DL-lactic-co-glycolic acid) (PLGA) and 75:25 PLGA were manufactured using a solvent casting technique. Human D407 RPE like cells were cultured on these films at a seeding density of 107,000 cells/cm2. The morphological and structural properties of the cells were investigated by light and electron microscopy. Results, Human D407 RPE like cells attached and proliferated on all the polymer substrates tested, comparable to the control tissue culture polystyrene. For example, 68.1{\%} of the cells plated attached after 8 hrs to 75:25 PLGA films. After 4 days in culture, the cells reached confluency with a density of 1,062,000 cells/cm2 and possessed normal epithelial cobblestone morphology. Conclusions. Biodegradable polymer films can provide suitable substrates for human RPE like cell culture, and hold promise as temporary carriers for subretinal implantation of organized sheets of RPE cells.",
author = "Lichun Lu and Thomson, {Robert C.} and Mikos, {Antonios G.} and Hunt, {Richard C.} and Giordano, {Giovanni G.} and Garcia, {Charles A.} and Devjani Lahiri-Munir",
year = "1996",
month = "2",
day = "15",
language = "English (US)",
volume = "37",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - Human RPE like cell culture on biodegradable polymer substrates

AU - Lu, Lichun

AU - Thomson, Robert C.

AU - Mikos, Antonios G.

AU - Hunt, Richard C.

AU - Giordano, Giovanni G.

AU - Garcia, Charles A.

AU - Lahiri-Munir, Devjani

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose. To evaluate the morphology, structure and differentiated function of cultured human D407 retinal pigment epithelium (RPE) like cells on synthetic biodegradable polymer substrates. Methods, Biodegradable polymer films of 50:50 poly(DL-lactic-co-glycolic acid) (PLGA) and 75:25 PLGA were manufactured using a solvent casting technique. Human D407 RPE like cells were cultured on these films at a seeding density of 107,000 cells/cm2. The morphological and structural properties of the cells were investigated by light and electron microscopy. Results, Human D407 RPE like cells attached and proliferated on all the polymer substrates tested, comparable to the control tissue culture polystyrene. For example, 68.1% of the cells plated attached after 8 hrs to 75:25 PLGA films. After 4 days in culture, the cells reached confluency with a density of 1,062,000 cells/cm2 and possessed normal epithelial cobblestone morphology. Conclusions. Biodegradable polymer films can provide suitable substrates for human RPE like cell culture, and hold promise as temporary carriers for subretinal implantation of organized sheets of RPE cells.

AB - Purpose. To evaluate the morphology, structure and differentiated function of cultured human D407 retinal pigment epithelium (RPE) like cells on synthetic biodegradable polymer substrates. Methods, Biodegradable polymer films of 50:50 poly(DL-lactic-co-glycolic acid) (PLGA) and 75:25 PLGA were manufactured using a solvent casting technique. Human D407 RPE like cells were cultured on these films at a seeding density of 107,000 cells/cm2. The morphological and structural properties of the cells were investigated by light and electron microscopy. Results, Human D407 RPE like cells attached and proliferated on all the polymer substrates tested, comparable to the control tissue culture polystyrene. For example, 68.1% of the cells plated attached after 8 hrs to 75:25 PLGA films. After 4 days in culture, the cells reached confluency with a density of 1,062,000 cells/cm2 and possessed normal epithelial cobblestone morphology. Conclusions. Biodegradable polymer films can provide suitable substrates for human RPE like cell culture, and hold promise as temporary carriers for subretinal implantation of organized sheets of RPE cells.

UR - http://www.scopus.com/inward/record.url?scp=33750153232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750153232&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750153232

VL - 37

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -