Human osteoblast-like cells express aromatase immunoreactivity

Lucia Roa-Peña, Tony Zreik, Nobuhiro Harada, Thomas C. Spelsberg, B. Lawrence Riggs, Frederick Naftolin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The main source of estrogen after menopause is the peripheral aromatization of adrenal androgens. Estrogen synthetase (aromatase) has been described in several tissues including placenta, ovary, testis, brain, and adipose tissue. The possibility that bone cells themselves could be a local source of estrogen production has been raised in previous studies that reported the presence of aromatase activity in crushed rat mandible and human bone cells. To determine the presence of aromatase in bone cells, cultured male and female human osteoblast-like cells were immunostained with a specific antiserum generated against human aromatase. Aromatase-immunopositive granules were found throughout the entire cytoplasm in all cultures. The morphological demonstration of immunoreactive aromatase in bone cells indicates that bone may be a site of extragonadal aromatization in both sexes. These findings open new possibilities for understanding the mechanisms of bone metabolism, the development of peak bone mass, sex differences in bone mass, and issues regarding the role of sex steroids in bone maintenance and repair.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalMenopause
Volume1
Issue number2
StatePublished - 1994

Fingerprint

Aromatase
Osteoblasts
Bone and Bones
Estrogens
Bone Development
Menopause
Mandible
Sex Characteristics
Placenta
Androgens
Adipose Tissue
Testis
Immune Sera
Ovary
Cultured Cells
Cytoplasm
Steroids
Maintenance
Brain

Keywords

  • Bone
  • Cell culture
  • Estrogen synthetase
  • Osteoporosis
  • Sex steroids

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Roa-Peña, L., Zreik, T., Harada, N., Spelsberg, T. C., Lawrence Riggs, B., & Naftolin, F. (1994). Human osteoblast-like cells express aromatase immunoreactivity. Menopause, 1(2), 73-78.

Human osteoblast-like cells express aromatase immunoreactivity. / Roa-Peña, Lucia; Zreik, Tony; Harada, Nobuhiro; Spelsberg, Thomas C.; Lawrence Riggs, B.; Naftolin, Frederick.

In: Menopause, Vol. 1, No. 2, 1994, p. 73-78.

Research output: Contribution to journalArticle

Roa-Peña, L, Zreik, T, Harada, N, Spelsberg, TC, Lawrence Riggs, B & Naftolin, F 1994, 'Human osteoblast-like cells express aromatase immunoreactivity', Menopause, vol. 1, no. 2, pp. 73-78.
Roa-Peña L, Zreik T, Harada N, Spelsberg TC, Lawrence Riggs B, Naftolin F. Human osteoblast-like cells express aromatase immunoreactivity. Menopause. 1994;1(2):73-78.
Roa-Peña, Lucia ; Zreik, Tony ; Harada, Nobuhiro ; Spelsberg, Thomas C. ; Lawrence Riggs, B. ; Naftolin, Frederick. / Human osteoblast-like cells express aromatase immunoreactivity. In: Menopause. 1994 ; Vol. 1, No. 2. pp. 73-78.
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