TY - JOUR
T1 - Human immunodeficiency virus type 2 (HIV-2) vector-mediated in vivo gene transfer into adult rabbit retina
AU - Cheng, Lingyun
AU - Chaidhawangul, Sunan
AU - Wong-Staal, Flossie
AU - Gilbert, James
AU - Poeschla, Eric
AU - Toyoguchi, Mitsuko
AU - El-Bradey, Mohamed H.
AU - Bergeron-Lynn, Germaine
AU - Soules, Kelly A.
AU - Freeman, William R.
N1 - Funding Information:
Supported in part by NIH grants EY-07366 (William Freeman) and AI-45992 (Flossie Wong-Staal).
PY - 2002
Y1 - 2002
N2 - Purpose. To evaluate the potential usefulness of HIV-2 viral vector in in vivo retinal gene therapy. Methods. An HIV-2 virus based viral vector was constructed and administered subretinally and intravitreally into rabbit eyes. After viral vector administration, the eyes were closely monitored for any adverse effects by slit lamp, indirect ophthalmoscopy, and fundus photography. Eyes were enucleated at specified times after injection, and reporter gene expression was identified within cell types and graded by the pattern and distribution of staining cells using fluorescent microscopy. Results. The HIV-2 viral vector demonstrated efficient gene transfer into many types of retinal cells without apparent cytotoxicity. Notably with subretinal injection, the HIV-2 vector resulted in higher efficiency of transduction of photoreceptor cells than of the other cell types (p < 0.05). With the intravitreal administration of HIV-2 viral vectors, cellular transduction and transgene expression in the ganglion cell layer was the dominant finding. Conclusions. HIV-2 viral vector may be a useful gene delivery vehicle for retinal photoreceptor cells and ganglion cells. It deserves further exploration to investigate its potential merit in long term gene therapy protocols and in other animal species.
AB - Purpose. To evaluate the potential usefulness of HIV-2 viral vector in in vivo retinal gene therapy. Methods. An HIV-2 virus based viral vector was constructed and administered subretinally and intravitreally into rabbit eyes. After viral vector administration, the eyes were closely monitored for any adverse effects by slit lamp, indirect ophthalmoscopy, and fundus photography. Eyes were enucleated at specified times after injection, and reporter gene expression was identified within cell types and graded by the pattern and distribution of staining cells using fluorescent microscopy. Results. The HIV-2 viral vector demonstrated efficient gene transfer into many types of retinal cells without apparent cytotoxicity. Notably with subretinal injection, the HIV-2 vector resulted in higher efficiency of transduction of photoreceptor cells than of the other cell types (p < 0.05). With the intravitreal administration of HIV-2 viral vectors, cellular transduction and transgene expression in the ganglion cell layer was the dominant finding. Conclusions. HIV-2 viral vector may be a useful gene delivery vehicle for retinal photoreceptor cells and ganglion cells. It deserves further exploration to investigate its potential merit in long term gene therapy protocols and in other animal species.
KW - GFP
KW - Gene therapy
KW - HIV-2 virus vector
KW - Rabbit
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U2 - 10.1076/ceyr.24.3.196.8302
DO - 10.1076/ceyr.24.3.196.8302
M3 - Article
C2 - 12221527
AN - SCOPUS:18644376567
SN - 0271-3683
VL - 24
SP - 196
EP - 201
JO - Current Eye Research
JF - Current Eye Research
IS - 3
ER -