TY - JOUR
T1 - Human chorionic somatomammotropin and growth hormone gene expression in rat pituitary tumour cells is dependent on proximal promoter sequences
AU - Nachtigal, Mark W.
AU - Nickel, Barbara E.
AU - Klassen, Margaret E.
AU - Zhang, Wengang
AU - Eberhardt, Norman L.
AU - Cattini, Peter A.
N1 - Funding Information:
This work was supported by grants from the Medical Council of Canada (MRC) and the National Institutes of B.E.N. is an MRC Fellow and P.A.C is an MRC Scholar.
PY - 1989/6/12
Y1 - 1989/6/12
N2 - Human placental chorionic somatomammotropin (hCS-A or hCS-B) and pituitary growth hormone (hGH-N) are related by structure and function. The hCS-A gene is expressed in rat pituitary tumour (GC) cells after gene transfer. Deletion of hCS-A 5′-flanking DNA reveals repressor activity upstream of nucleotide -132, and a region essential for expression in GC cells between nucleotides -94 and -61. The sequences in this region differ from the equivalent hGH-N gene DNA by one nucleotide, and include the binding site (-92 to -65) for a pituitary-specific factor (GHF-1), required for hGH-N expression in GC cells. Exchange of hGH-N with hCS-A gene DNA in this region maintains expression in GC cells. By contrast, modification of these sequences blocks expression. These data indicate that proximal promoter sequences, equivalent to those bound by GHF-1 on the hGH-N gene, are required for hCS-A expression in GC cells.
AB - Human placental chorionic somatomammotropin (hCS-A or hCS-B) and pituitary growth hormone (hGH-N) are related by structure and function. The hCS-A gene is expressed in rat pituitary tumour (GC) cells after gene transfer. Deletion of hCS-A 5′-flanking DNA reveals repressor activity upstream of nucleotide -132, and a region essential for expression in GC cells between nucleotides -94 and -61. The sequences in this region differ from the equivalent hGH-N gene DNA by one nucleotide, and include the binding site (-92 to -65) for a pituitary-specific factor (GHF-1), required for hGH-N expression in GC cells. Exchange of hGH-N with hCS-A gene DNA in this region maintains expression in GC cells. By contrast, modification of these sequences blocks expression. These data indicate that proximal promoter sequences, equivalent to those bound by GHF-1 on the hGH-N gene, are required for hCS-A expression in GC cells.
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U2 - 10.1093/nar/17.11.4327
DO - 10.1093/nar/17.11.4327
M3 - Article
C2 - 2740217
AN - SCOPUS:0024346518
SN - 0305-1048
VL - 17
SP - 4327
EP - 4337
JO - Nucleic acids research
JF - Nucleic acids research
IS - 11
ER -