Human acid ceramidase is overexpressed but not mutated in prostate cancer

Ratnam S. Seelan, Chiping Qian, Akira Yokomizo, David G. Bostwick, David I Smith, Wanguo Liu

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

The human acid ceramidase gene, that causes Farber disease, is located in 8p22, a region frequently altered in several cancers, including prostate cancer. Acid ceramidase catalyzes the hydrolysis of ceramide, a potent lipid second messenger molecule that promotes apoptosis and inhibits cellular proliferation. It is not known whether this gene, or its expression, is altered in prostate cancer. Here, we report the structural organization of the human gene, its expression in human tissues, and the identification of several single nucleotide polymorphisms. No cancer-related mutations were found in the gene in a panel of prostate tumor DNAs analyzed, but increased expression was observed in prostate tumor tissues when compared with matched normals. This increase was observed in all three prostate tumor cell lines tested (DU 145, LnCAP, and PC3) when compared to a BPH (benign prostatic hyperplasia) cell line and 15/36 prostate tumors. These results suggest that acid ceramidase may play an important role in prostate carcinogenesis. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalGenes Chromosomes and Cancer
Volume29
Issue number2
DOIs
StatePublished - Oct 2000

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Prostate
Prostatic Neoplasms
Acid Ceramidase
Farber Lipogranulomatosis
Neoplasms
Genes
Forensic Anthropology
Ceramides
Prostatic Hyperplasia
Second Messenger Systems
Tumor Cell Line
Single Nucleotide Polymorphism
Carcinogenesis
Hydrolysis
Cell Proliferation
human ASAH1 protein
Apoptosis
Lipids
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Human acid ceramidase is overexpressed but not mutated in prostate cancer. / Seelan, Ratnam S.; Qian, Chiping; Yokomizo, Akira; Bostwick, David G.; Smith, David I; Liu, Wanguo.

In: Genes Chromosomes and Cancer, Vol. 29, No. 2, 10.2000, p. 137-146.

Research output: Contribution to journalArticle

Seelan, Ratnam S. ; Qian, Chiping ; Yokomizo, Akira ; Bostwick, David G. ; Smith, David I ; Liu, Wanguo. / Human acid ceramidase is overexpressed but not mutated in prostate cancer. In: Genes Chromosomes and Cancer. 2000 ; Vol. 29, No. 2. pp. 137-146.
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