TY - JOUR
T1 - Human acid ceramidase is overexpressed but not mutated in prostate cancer
AU - Seelan, Ratnam S.
AU - Qian, Chiping
AU - Yokomizo, Akira
AU - Bostwick, David G.
AU - Smith, David I.
AU - Liu, Wanguo
PY - 2000/10
Y1 - 2000/10
N2 - The human acid ceramidase gene, that causes Farber disease, is located in 8p22, a region frequently altered in several cancers, including prostate cancer. Acid ceramidase catalyzes the hydrolysis of ceramide, a potent lipid second messenger molecule that promotes apoptosis and inhibits cellular proliferation. It is not known whether this gene, or its expression, is altered in prostate cancer. Here, we report the structural organization of the human gene, its expression in human tissues, and the identification of several single nucleotide polymorphisms. No cancer-related mutations were found in the gene in a panel of prostate tumor DNAs analyzed, but increased expression was observed in prostate tumor tissues when compared with matched normals. This increase was observed in all three prostate tumor cell lines tested (DU 145, LnCAP, and PC3) when compared to a BPH (benign prostatic hyperplasia) cell line and 15/36 prostate tumors. These results suggest that acid ceramidase may play an important role in prostate carcinogenesis. (C) 2000 Wiley-Liss, Inc.
AB - The human acid ceramidase gene, that causes Farber disease, is located in 8p22, a region frequently altered in several cancers, including prostate cancer. Acid ceramidase catalyzes the hydrolysis of ceramide, a potent lipid second messenger molecule that promotes apoptosis and inhibits cellular proliferation. It is not known whether this gene, or its expression, is altered in prostate cancer. Here, we report the structural organization of the human gene, its expression in human tissues, and the identification of several single nucleotide polymorphisms. No cancer-related mutations were found in the gene in a panel of prostate tumor DNAs analyzed, but increased expression was observed in prostate tumor tissues when compared with matched normals. This increase was observed in all three prostate tumor cell lines tested (DU 145, LnCAP, and PC3) when compared to a BPH (benign prostatic hyperplasia) cell line and 15/36 prostate tumors. These results suggest that acid ceramidase may play an important role in prostate carcinogenesis. (C) 2000 Wiley-Liss, Inc.
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U2 - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1018>3.0.CO;2-E
DO - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1018>3.0.CO;2-E
M3 - Article
C2 - 10959093
AN - SCOPUS:0033839592
SN - 1045-2257
VL - 29
SP - 137
EP - 146
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
IS - 2
ER -