Household endotoxin levels and the risk of non-Hodgkin lymphoma

Jun Wang, Wendy Cozen, Peter S. Thorne, Kiros Berhane, James R Cerhan, Patricia Hartge, Mary H. Ward, Anneclaire J. De Roos, Richard K. Severson, Lindsay M. Morton, Leslie Bernstein, Martha S. Linet, Joanne S. Colt

Research output: Contribution to journalArticle

Abstract

Objective: Endotoxin, a component of the outer membrane of gram-negative bacteria, elicits a strong innate and inflammatory immune response associated with the secretion of proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-α). Because TNF-α polymorphisms that increase TNF-α production are associated with an increased risk of non-Hodgkin lymphoma (NHL), we hypothesized that increased levels of household endotoxin would be associated with an increased NHL risk. Methods: We evaluated this association in the National Cancer Institute/Surveillance, Epidemiology and End Results (NCI/SEER) NHL multicenter population-based case-control study. Used vacuum cleaner bags were collected from participants during a home interview. Dust samples from the bags of 594 cases and 442 controls were analyzed for endotoxin [endotoxin unit (EU)/mg of dust] using the kinetic chromogenic Limulus amebocyte lysate assay. Multivariable logistic regression was used to estimate the effect of endotoxin on NHL risk adjusted for age, sex, race, education, study center, and farm exposure. Results: Endotoxin was not associated with NHL overall [odds ratio (OR) for highest quartile of endotoxin levels = 0.81, 95 % confidence interval (CI) = 0.55, 1.20; p for trend = 0.35] or with diffuse large B-cell lymphoma (OR = 0.63, 95 % CI = 0.34, 1.16; p = 0.31) or follicular lymphoma (OR = 1.07, 95 % CI = 0.61, 1.89; p = 0.73) subtypes. Both working and living on a farm were associated with higher household endotoxin levels compared to never working (p = 0.009) or living (p = 0.01) on a farm. Excluding farmers from the analysis did not change the results. Conclusions: We found no evidence of a role for household endotoxin in NHL etiology.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalCancer Causes and Control
Volume24
Issue number2
DOIs
StatePublished - Feb 2013

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Endotoxins
Non-Hodgkin's Lymphoma
Tumor Necrosis Factor-alpha
Odds Ratio
Confidence Intervals
Dust
Horseshoe Crabs
Follicular Lymphoma
Sex Education
National Cancer Institute (U.S.)
Lymphoma, Large B-Cell, Diffuse
Vacuum
Gram-Negative Bacteria
Innate Immunity
Case-Control Studies
Epidemiology
Logistic Models
Interviews
Cytokines
Membranes

Keywords

  • Case-control
  • Endotoxin
  • Epidemiology
  • Farming
  • Non-Hodgkin lymphoma
  • Risk

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Wang, J., Cozen, W., Thorne, P. S., Berhane, K., Cerhan, J. R., Hartge, P., ... Colt, J. S. (2013). Household endotoxin levels and the risk of non-Hodgkin lymphoma. Cancer Causes and Control, 24(2), 357-364. https://doi.org/10.1007/s10552-012-0121-9

Household endotoxin levels and the risk of non-Hodgkin lymphoma. / Wang, Jun; Cozen, Wendy; Thorne, Peter S.; Berhane, Kiros; Cerhan, James R; Hartge, Patricia; Ward, Mary H.; De Roos, Anneclaire J.; Severson, Richard K.; Morton, Lindsay M.; Bernstein, Leslie; Linet, Martha S.; Colt, Joanne S.

In: Cancer Causes and Control, Vol. 24, No. 2, 02.2013, p. 357-364.

Research output: Contribution to journalArticle

Wang, J, Cozen, W, Thorne, PS, Berhane, K, Cerhan, JR, Hartge, P, Ward, MH, De Roos, AJ, Severson, RK, Morton, LM, Bernstein, L, Linet, MS & Colt, JS 2013, 'Household endotoxin levels and the risk of non-Hodgkin lymphoma', Cancer Causes and Control, vol. 24, no. 2, pp. 357-364. https://doi.org/10.1007/s10552-012-0121-9
Wang, Jun ; Cozen, Wendy ; Thorne, Peter S. ; Berhane, Kiros ; Cerhan, James R ; Hartge, Patricia ; Ward, Mary H. ; De Roos, Anneclaire J. ; Severson, Richard K. ; Morton, Lindsay M. ; Bernstein, Leslie ; Linet, Martha S. ; Colt, Joanne S. / Household endotoxin levels and the risk of non-Hodgkin lymphoma. In: Cancer Causes and Control. 2013 ; Vol. 24, No. 2. pp. 357-364.
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abstract = "Objective: Endotoxin, a component of the outer membrane of gram-negative bacteria, elicits a strong innate and inflammatory immune response associated with the secretion of proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-α). Because TNF-α polymorphisms that increase TNF-α production are associated with an increased risk of non-Hodgkin lymphoma (NHL), we hypothesized that increased levels of household endotoxin would be associated with an increased NHL risk. Methods: We evaluated this association in the National Cancer Institute/Surveillance, Epidemiology and End Results (NCI/SEER) NHL multicenter population-based case-control study. Used vacuum cleaner bags were collected from participants during a home interview. Dust samples from the bags of 594 cases and 442 controls were analyzed for endotoxin [endotoxin unit (EU)/mg of dust] using the kinetic chromogenic Limulus amebocyte lysate assay. Multivariable logistic regression was used to estimate the effect of endotoxin on NHL risk adjusted for age, sex, race, education, study center, and farm exposure. Results: Endotoxin was not associated with NHL overall [odds ratio (OR) for highest quartile of endotoxin levels = 0.81, 95 {\%} confidence interval (CI) = 0.55, 1.20; p for trend = 0.35] or with diffuse large B-cell lymphoma (OR = 0.63, 95 {\%} CI = 0.34, 1.16; p = 0.31) or follicular lymphoma (OR = 1.07, 95 {\%} CI = 0.61, 1.89; p = 0.73) subtypes. Both working and living on a farm were associated with higher household endotoxin levels compared to never working (p = 0.009) or living (p = 0.01) on a farm. Excluding farmers from the analysis did not change the results. Conclusions: We found no evidence of a role for household endotoxin in NHL etiology.",
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AU - Cozen, Wendy

AU - Thorne, Peter S.

AU - Berhane, Kiros

AU - Cerhan, James R

AU - Hartge, Patricia

AU - Ward, Mary H.

AU - De Roos, Anneclaire J.

AU - Severson, Richard K.

AU - Morton, Lindsay M.

AU - Bernstein, Leslie

AU - Linet, Martha S.

AU - Colt, Joanne S.

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N2 - Objective: Endotoxin, a component of the outer membrane of gram-negative bacteria, elicits a strong innate and inflammatory immune response associated with the secretion of proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-α). Because TNF-α polymorphisms that increase TNF-α production are associated with an increased risk of non-Hodgkin lymphoma (NHL), we hypothesized that increased levels of household endotoxin would be associated with an increased NHL risk. Methods: We evaluated this association in the National Cancer Institute/Surveillance, Epidemiology and End Results (NCI/SEER) NHL multicenter population-based case-control study. Used vacuum cleaner bags were collected from participants during a home interview. Dust samples from the bags of 594 cases and 442 controls were analyzed for endotoxin [endotoxin unit (EU)/mg of dust] using the kinetic chromogenic Limulus amebocyte lysate assay. Multivariable logistic regression was used to estimate the effect of endotoxin on NHL risk adjusted for age, sex, race, education, study center, and farm exposure. Results: Endotoxin was not associated with NHL overall [odds ratio (OR) for highest quartile of endotoxin levels = 0.81, 95 % confidence interval (CI) = 0.55, 1.20; p for trend = 0.35] or with diffuse large B-cell lymphoma (OR = 0.63, 95 % CI = 0.34, 1.16; p = 0.31) or follicular lymphoma (OR = 1.07, 95 % CI = 0.61, 1.89; p = 0.73) subtypes. Both working and living on a farm were associated with higher household endotoxin levels compared to never working (p = 0.009) or living (p = 0.01) on a farm. Excluding farmers from the analysis did not change the results. Conclusions: We found no evidence of a role for household endotoxin in NHL etiology.

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