Abstract
Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (β-S)triphosphates. Initiation of pre-rRNA was completely resistant to α-amanitin but was inhibited by either actinomycin D or heparin. Experiments with P1798 mouse lymphoma cells indicated that the antiproliferative effects of glucocorticoids on lymphoid cells includes a reduction in the ability of nuclei to initiate pre-rRNA chains.
Original language | English (US) |
---|---|
Pages (from-to) | 8115-8128 |
Number of pages | 14 |
Journal | Nucleic acids research |
Volume | 12 |
Issue number | 21 |
DOIs | |
State | Published - Nov 12 1984 |
ASJC Scopus subject areas
- Genetics