Hormonal regulation of transcription of rDNA: Use of nucleoside thiotriphosphates to measure initiation in isolated nuclei

Zhen Yong Zhang, E. Aubrey Thompson, Michael R. Stallcup

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (β-S)triphosphates. Initiation of pre-rRNA was completely resistant to α-amanitin but was inhibited by either actinomycin D or heparin. Experiments with P1798 mouse lymphoma cells indicated that the antiproliferative effects of glucocorticoids on lymphoid cells includes a reduction in the ability of nuclei to initiate pre-rRNA chains.

Original languageEnglish (US)
Pages (from-to)8115-8128
Number of pages14
JournalNucleic acids research
Volume12
Issue number21
DOIs
StatePublished - Nov 12 1984

ASJC Scopus subject areas

  • Genetics

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