Homonuclear two-dimensional 1H NMR of proteins. Experimental procedures

G. Wider, Slobodan I Macura, Anil Kumar, R. R. Ernst, K. Wüthrich

Research output: Contribution to journalArticle

378 Citations (Scopus)

Abstract

Experimental techniques used for homonuclear 2D 1H NMR studies of proteins are described. A brief survey of the general strategy for structural studies of proteins by 2D NMR is included. The main part of the paper discusses guidelines for the selection of experimental techniques, the elimination of artifacts and unwanted peaks in protein 2D 1H NMR spectra, suppression of the solvent line in H2O solutions, experimental parameters, numerical data processing before and after Fourier transformation, and suitable presentations of complex 2D NMR spectra.

Original languageEnglish (US)
Pages (from-to)207-234
Number of pages28
JournalJournal of Magnetic Resonance (1969)
Volume56
Issue number2
DOIs
StatePublished - Feb 1 1984
Externally publishedYes

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Nuclear magnetic resonance
Proteins

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Homonuclear two-dimensional 1H NMR of proteins. Experimental procedures. / Wider, G.; Macura, Slobodan I; Kumar, Anil; Ernst, R. R.; Wüthrich, K.

In: Journal of Magnetic Resonance (1969), Vol. 56, No. 2, 01.02.1984, p. 207-234.

Research output: Contribution to journalArticle

Wider, G. ; Macura, Slobodan I ; Kumar, Anil ; Ernst, R. R. ; Wüthrich, K. / Homonuclear two-dimensional 1H NMR of proteins. Experimental procedures. In: Journal of Magnetic Resonance (1969). 1984 ; Vol. 56, No. 2. pp. 207-234.
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