TY - JOUR
T1 - Homonuclear two-dimensional 1H NMR of proteins. Experimental procedures
AU - Wider, G.
AU - Macura, S.
AU - Kumar, Anil
AU - Ernst, R. R.
AU - Wüthrich, K.
N1 - Funding Information:
Financial support by special grants of the Eidgenossische Technische Hochschule, Zurich, and by the Schweizerischer Nationalfonds (Project 3.528.79) are gratefully acknowledged. The authors are grateful to Dr. G. Bodenhausen and Dr. M. P. Williamson for helpful suggestions with regard to the manuscript, to Mr. A. Eugster for recording the spectra of Fig. 11, to Mrs. Y. Hunziker for drawing some of the &gums and to Mrs. I. Kowalski (Spectrospin AG, Fallanden) for typing the manuscript. The authors also acknowledge the support by Spectrospin AG, Fallanden.
PY - 1984/2/1
Y1 - 1984/2/1
N2 - Experimental techniques used for homonuclear 2D 1H NMR studies of proteins are described. A brief survey of the general strategy for structural studies of proteins by 2D NMR is included. The main part of the paper discusses guidelines for the selection of experimental techniques, the elimination of artifacts and unwanted peaks in protein 2D 1H NMR spectra, suppression of the solvent line in H2O solutions, experimental parameters, numerical data processing before and after Fourier transformation, and suitable presentations of complex 2D NMR spectra.
AB - Experimental techniques used for homonuclear 2D 1H NMR studies of proteins are described. A brief survey of the general strategy for structural studies of proteins by 2D NMR is included. The main part of the paper discusses guidelines for the selection of experimental techniques, the elimination of artifacts and unwanted peaks in protein 2D 1H NMR spectra, suppression of the solvent line in H2O solutions, experimental parameters, numerical data processing before and after Fourier transformation, and suitable presentations of complex 2D NMR spectra.
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U2 - 10.1016/0022-2364(84)90099-4
DO - 10.1016/0022-2364(84)90099-4
M3 - Article
AN - SCOPUS:0001129286
SN - 0022-2364
VL - 56
SP - 207
EP - 234
JO - Journal of Magnetic Resonance (1969)
JF - Journal of Magnetic Resonance (1969)
IS - 2
ER -