HLA homozygosity does not adversely affect measles vaccine-induced cytokine responses

Inna G. Ovsyannikova; Robert M. Jacobson; Neelam Dhiman; Robert A. Vierkant; V. Shane Pankratz; Gregory A. Poland

doi:10.1016/j.virol.2007.02.028

HLA homozygosity does not adversely affect measles vaccine-induced cytokine responses

Inna G. Ovsyannikova, Robert M. Jacobson, Neelam Dhiman, Robert A. Vierkant, V. Shane Pankratz, Gregory A. Poland

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The association between HLA homozygosity and measles-specific Th₁ (IFN-γ, IL-2 and IL-12p40) and Th₂ (IL-4 and IL-10) cytokine responses were assessed in a group of 339 healthy schoolchildren 12-18 years of age previously immunized with two doses of live-attenuated measles virus vaccine. No associations were observed between class I HLA homozygosity and measles-specific cytokine levels. Children who were homozygous at the class II DRB1, DQA1, DPA1 and DPB1 loci had higher median IFN-γ secretion levels compared with children who were heterozygous for DRB1 (77.7 vs. 39.5 pg/ml, p = 0.05), DQA1 (60.9 vs. 36.6 pg/ml, p = 0.03), DPA1 (46.1 vs. 27.1 pg/ml, p = 0.01) and DPB1 (61.5 vs. 36.0 pg/ml, p = 0.01) loci, respectively. Homozygosity at increasing numbers of HLA loci ( >= 4) was associated with increased IFN-γ secretion levels (test for trend p-value = 0.01). Our results suggest that HLA homozygosity showed no disadvantage for measles-specific cytokine responses and instead was associated with increased IFN-γ levels.

Original language	English (US)
Pages (from-to)	87-94
Number of pages	8
Journal	Virology
Volume	364
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2007.02.028
State	Published - Jul 20 2007

Keywords

Cytokines
HLA
Homozygosity
Measles vaccine
Measles virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2007.02.028

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title = "HLA homozygosity does not adversely affect measles vaccine-induced cytokine responses",

abstract = "The association between HLA homozygosity and measles-specific Th1 (IFN-γ, IL-2 and IL-12p40) and Th2 (IL-4 and IL-10) cytokine responses were assessed in a group of 339 healthy schoolchildren 12-18 years of age previously immunized with two doses of live-attenuated measles virus vaccine. No associations were observed between class I HLA homozygosity and measles-specific cytokine levels. Children who were homozygous at the class II DRB1, DQA1, DPA1 and DPB1 loci had higher median IFN-γ secretion levels compared with children who were heterozygous for DRB1 (77.7 vs. 39.5 pg/ml, p = 0.05), DQA1 (60.9 vs. 36.6 pg/ml, p = 0.03), DPA1 (46.1 vs. 27.1 pg/ml, p = 0.01) and DPB1 (61.5 vs. 36.0 pg/ml, p = 0.01) loci, respectively. Homozygosity at increasing numbers of HLA loci ( >= 4) was associated with increased IFN-γ secretion levels (test for trend p-value = 0.01). Our results suggest that HLA homozygosity showed no disadvantage for measles-specific cytokine responses and instead was associated with increased IFN-γ levels.",

keywords = "Cytokines, HLA, Homozygosity, Measles vaccine, Measles virus",

author = "Ovsyannikova, {Inna G.} and Jacobson, {Robert M.} and Neelam Dhiman and Vierkant, {Robert A.} and Pankratz, {V. Shane} and Poland, {Gregory A.}",

note = "Funding Information: We thank the parents and children who participated in this study. We acknowledge the efforts of the fellows, nurses, and technicians from the Mayo Vaccine Research Group. We thank Jenna E. Ryan and Norman A. Pinsky for performing the cytokine secretion assays and Leslie Sutherland and Tina A. Agostini for performing HLA typing. We thank Carla L. Tentis for her editorial assistance in preparing this manuscript. This study was supported by NIH grants AI 33144 and AI 48793, and Mayo Clinic General Clinical Research Center grant MO1 RR00585. Dr. Gregory A. Poland chairs a DMSB for clinical trials of new vaccines sponsored by Merck Research Laboratories – but none involve measles vaccines.",

year = "2007",

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doi = "10.1016/j.virol.2007.02.028",

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T1 - HLA homozygosity does not adversely affect measles vaccine-induced cytokine responses

AU - Ovsyannikova, Inna G.

AU - Jacobson, Robert M.

AU - Dhiman, Neelam

AU - Vierkant, Robert A.

AU - Pankratz, V. Shane

AU - Poland, Gregory A.

N1 - Funding Information: We thank the parents and children who participated in this study. We acknowledge the efforts of the fellows, nurses, and technicians from the Mayo Vaccine Research Group. We thank Jenna E. Ryan and Norman A. Pinsky for performing the cytokine secretion assays and Leslie Sutherland and Tina A. Agostini for performing HLA typing. We thank Carla L. Tentis for her editorial assistance in preparing this manuscript. This study was supported by NIH grants AI 33144 and AI 48793, and Mayo Clinic General Clinical Research Center grant MO1 RR00585. Dr. Gregory A. Poland chairs a DMSB for clinical trials of new vaccines sponsored by Merck Research Laboratories – but none involve measles vaccines.

PY - 2007/7/20

Y1 - 2007/7/20

N2 - The association between HLA homozygosity and measles-specific Th1 (IFN-γ, IL-2 and IL-12p40) and Th2 (IL-4 and IL-10) cytokine responses were assessed in a group of 339 healthy schoolchildren 12-18 years of age previously immunized with two doses of live-attenuated measles virus vaccine. No associations were observed between class I HLA homozygosity and measles-specific cytokine levels. Children who were homozygous at the class II DRB1, DQA1, DPA1 and DPB1 loci had higher median IFN-γ secretion levels compared with children who were heterozygous for DRB1 (77.7 vs. 39.5 pg/ml, p = 0.05), DQA1 (60.9 vs. 36.6 pg/ml, p = 0.03), DPA1 (46.1 vs. 27.1 pg/ml, p = 0.01) and DPB1 (61.5 vs. 36.0 pg/ml, p = 0.01) loci, respectively. Homozygosity at increasing numbers of HLA loci ( >= 4) was associated with increased IFN-γ secretion levels (test for trend p-value = 0.01). Our results suggest that HLA homozygosity showed no disadvantage for measles-specific cytokine responses and instead was associated with increased IFN-γ levels.

AB - The association between HLA homozygosity and measles-specific Th1 (IFN-γ, IL-2 and IL-12p40) and Th2 (IL-4 and IL-10) cytokine responses were assessed in a group of 339 healthy schoolchildren 12-18 years of age previously immunized with two doses of live-attenuated measles virus vaccine. No associations were observed between class I HLA homozygosity and measles-specific cytokine levels. Children who were homozygous at the class II DRB1, DQA1, DPA1 and DPB1 loci had higher median IFN-γ secretion levels compared with children who were heterozygous for DRB1 (77.7 vs. 39.5 pg/ml, p = 0.05), DQA1 (60.9 vs. 36.6 pg/ml, p = 0.03), DPA1 (46.1 vs. 27.1 pg/ml, p = 0.01) and DPB1 (61.5 vs. 36.0 pg/ml, p = 0.01) loci, respectively. Homozygosity at increasing numbers of HLA loci ( >= 4) was associated with increased IFN-γ secretion levels (test for trend p-value = 0.01). Our results suggest that HLA homozygosity showed no disadvantage for measles-specific cytokine responses and instead was associated with increased IFN-γ levels.

KW - Cytokines

KW - HLA

KW - Homozygosity

KW - Measles vaccine

KW - Measles virus

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DO - 10.1016/j.virol.2007.02.028

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C2 - 17397893

AN - SCOPUS:34249709527

SN - 0042-6822

VL - 364

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EP - 94

JO - Virology

JF - Virology

IS - 1

ER -

HLA homozygosity does not adversely affect measles vaccine-induced cytokine responses

Abstract

Keywords

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