This study addresses whether hippocampal progenitor cells express nestin following cerebral ischemia in rats. Cell counts within the hippocampal hilus were significantly greater following severe (eight-vessel occlusion) ischemia than following intermediate (four-vessel occlusion) ischemia (1527 ± 87/mm2 vs. 918 ± 71/mm2). Bromedeoxyuridine- positive cell counts were significantly higher with severe ischemia than with intermediate ischemia or in sham-operated animals, respectively (368 ± 45, 43 ± 14 and 7 ± 1/mm2). In the eight-vessel occlusion group, 47 ± 8/mm2 bromedeoxyuridine-labeled cells expressed nestin, significantly higher than in the four-vessel occlusion group and sham-operated animals (1 ± 1 and 1 ± 0/mm2, P < 0.01 vs. eight-vessel occlusion, respectively). Confocal microscopy verified that a subset of the bromedeoxyuridine-positive cells expressed nestin. In conclusion, severe ischemia elicits nestin expression in hippocampal progenitor cells in rats.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Sep 28 2005|
- Bromedeoxyuridine labeling
- Global ischemia
- Hippocampal progenitor cells
ASJC Scopus subject areas