Hippocampal progenitor cells express nestin following cerebral ischemia in rats

Zhen He; Li Cui; James F. Meschia; Dennis W. Dickson; Thomas G. Brott; James W. Simpkins; Arthur L. Day; Michael McKinney

doi:10.1097/01.wnr.0000179074.32035.46

Hippocampal progenitor cells express nestin following cerebral ischemia in rats

Zhen He, Li Cui, James F. Meschia, Dennis W. Dickson, Thomas G. Brott, James W. Simpkins, Arthur L. Day, Michael McKinney

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

This study addresses whether hippocampal progenitor cells express nestin following cerebral ischemia in rats. Cell counts within the hippocampal hilus were significantly greater following severe (eight-vessel occlusion) ischemia than following intermediate (four-vessel occlusion) ischemia (1527 ± 87/mm² vs. 918 ± 71/mm²). Bromedeoxyuridine- positive cell counts were significantly higher with severe ischemia than with intermediate ischemia or in sham-operated animals, respectively (368 ± 45, 43 ± 14 and 7 ± 1/mm²). In the eight-vessel occlusion group, 47 ± 8/mm² bromedeoxyuridine-labeled cells expressed nestin, significantly higher than in the four-vessel occlusion group and sham-operated animals (1 ± 1 and 1 ± 0/mm², P < 0.01 vs. eight-vessel occlusion, respectively). Confocal microscopy verified that a subset of the bromedeoxyuridine-positive cells expressed nestin. In conclusion, severe ischemia elicits nestin expression in hippocampal progenitor cells in rats.

Original language	English (US)
Pages (from-to)	1541-1544
Number of pages	4
Journal	NeuroReport
Volume	16
Issue number	14
DOIs	https://doi.org/10.1097/01.wnr.0000179074.32035.46
State	Published - Sep 28 2005

Keywords

Bromedeoxyuridine labeling
Global ischemia
Hippocampal progenitor cells
Nestin
Rat

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/01.wnr.0000179074.32035.46

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title = "Hippocampal progenitor cells express nestin following cerebral ischemia in rats",

abstract = "This study addresses whether hippocampal progenitor cells express nestin following cerebral ischemia in rats. Cell counts within the hippocampal hilus were significantly greater following severe (eight-vessel occlusion) ischemia than following intermediate (four-vessel occlusion) ischemia (1527 ± 87/mm2 vs. 918 ± 71/mm2). Bromedeoxyuridine- positive cell counts were significantly higher with severe ischemia than with intermediate ischemia or in sham-operated animals, respectively (368 ± 45, 43 ± 14 and 7 ± 1/mm2). In the eight-vessel occlusion group, 47 ± 8/mm2 bromedeoxyuridine-labeled cells expressed nestin, significantly higher than in the four-vessel occlusion group and sham-operated animals (1 ± 1 and 1 ± 0/mm2, P < 0.01 vs. eight-vessel occlusion, respectively). Confocal microscopy verified that a subset of the bromedeoxyuridine-positive cells expressed nestin. In conclusion, severe ischemia elicits nestin expression in hippocampal progenitor cells in rats.",

keywords = "Bromedeoxyuridine labeling, Global ischemia, Hippocampal progenitor cells, Nestin, Rat",

author = "Zhen He and Li Cui and Meschia, {James F.} and Dickson, {Dennis W.} and Brott, {Thomas G.} and Simpkins, {James W.} and Day, {Arthur L.} and Michael McKinney",

year = "2005",

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doi = "10.1097/01.wnr.0000179074.32035.46",

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AU - He, Zhen

AU - Cui, Li

AU - Meschia, James F.

AU - Dickson, Dennis W.

AU - Brott, Thomas G.

AU - Simpkins, James W.

AU - Day, Arthur L.

AU - McKinney, Michael

PY - 2005/9/28

Y1 - 2005/9/28

N2 - This study addresses whether hippocampal progenitor cells express nestin following cerebral ischemia in rats. Cell counts within the hippocampal hilus were significantly greater following severe (eight-vessel occlusion) ischemia than following intermediate (four-vessel occlusion) ischemia (1527 ± 87/mm2 vs. 918 ± 71/mm2). Bromedeoxyuridine- positive cell counts were significantly higher with severe ischemia than with intermediate ischemia or in sham-operated animals, respectively (368 ± 45, 43 ± 14 and 7 ± 1/mm2). In the eight-vessel occlusion group, 47 ± 8/mm2 bromedeoxyuridine-labeled cells expressed nestin, significantly higher than in the four-vessel occlusion group and sham-operated animals (1 ± 1 and 1 ± 0/mm2, P < 0.01 vs. eight-vessel occlusion, respectively). Confocal microscopy verified that a subset of the bromedeoxyuridine-positive cells expressed nestin. In conclusion, severe ischemia elicits nestin expression in hippocampal progenitor cells in rats.

AB - This study addresses whether hippocampal progenitor cells express nestin following cerebral ischemia in rats. Cell counts within the hippocampal hilus were significantly greater following severe (eight-vessel occlusion) ischemia than following intermediate (four-vessel occlusion) ischemia (1527 ± 87/mm2 vs. 918 ± 71/mm2). Bromedeoxyuridine- positive cell counts were significantly higher with severe ischemia than with intermediate ischemia or in sham-operated animals, respectively (368 ± 45, 43 ± 14 and 7 ± 1/mm2). In the eight-vessel occlusion group, 47 ± 8/mm2 bromedeoxyuridine-labeled cells expressed nestin, significantly higher than in the four-vessel occlusion group and sham-operated animals (1 ± 1 and 1 ± 0/mm2, P < 0.01 vs. eight-vessel occlusion, respectively). Confocal microscopy verified that a subset of the bromedeoxyuridine-positive cells expressed nestin. In conclusion, severe ischemia elicits nestin expression in hippocampal progenitor cells in rats.

KW - Bromedeoxyuridine labeling

KW - Global ischemia

KW - Hippocampal progenitor cells

KW - Nestin

KW - Rat

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Hippocampal progenitor cells express nestin following cerebral ischemia in rats

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