High cell surface death receptor expression determines type I Versus type II signaling

Xue Wei Meng, Kevin L. Peterson, Haiming Dai, Paula Schneider, Sun Hee Lee, Jin San Zhang, Alexander Koenig, Steve Bronk, Daniel D Billadeau, Gregory James Gores, Scott H Kaufmann

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Previous studies have suggested that there are two signaling pathways leading from ligation of the Fas receptor to induction of apoptosis. Type I signaling involves Fas ligand-induced recruitment of large amounts of FADD (FAS-associated death domain protein) and procaspase 8, leading to direct activation of caspase 3, whereas type II signaling involves Bid-mediated mitochondrial perturbation to amplify a more modest death receptor- initiated signal. The biochemical basis for this dichotomy has previously been unclear. Here we show that type I cells have a longer half-life for Fas message and express higher amounts of cell surface Fas, explaining the increased recruitment of FADD and subsequent signaling. Moreover, we demonstrate that cells with type II Fas signaling (Jurkat or HCT-15) can signal through a type I pathway upon forced receptor overexpression and that shRNA-mediated Fas down-regulation converts cells with type I signaling (A498) to type II signaling. Importantly, the same cells can exhibit type I signaling for Fas and type II signaling for TRAIL (TNF-α-related apoptosis-inducing ligand), indicating that the choice of signaling pathway is related to the specific receptor, not some other cellular feature. Additional experiments revealed that up-regulation of cell surface death receptor 5 levels by treatment with 7-ethyl-10-hydroxy- camptothecin converted TRAIL signaling in HCT116 cells from type II to type I. Collectively, these results suggest that the type I/type II dichotomy reflects differences in cell surface death receptor expression.

Original languageEnglish (US)
Pages (from-to)35823-35833
Number of pages11
JournalJournal of Biological Chemistry
Volume286
Issue number41
DOIs
StatePublished - Oct 14 2011

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Death Domain Receptors
Cell Surface Receptors
Cell Death
TNF-Related Apoptosis-Inducing Ligand
TNF-Related Apoptosis-Inducing Ligand Receptors
HCT116 Cells
CD95 Antigens
Camptothecin
Fas Ligand Protein
Caspase 8
Caspase 3
Small Interfering RNA
Ligation
Half-Life
Proteins
Up-Regulation
Down-Regulation
Apoptosis
Chemical activation
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

High cell surface death receptor expression determines type I Versus type II signaling. / Meng, Xue Wei; Peterson, Kevin L.; Dai, Haiming; Schneider, Paula; Lee, Sun Hee; Zhang, Jin San; Koenig, Alexander; Bronk, Steve; Billadeau, Daniel D; Gores, Gregory James; Kaufmann, Scott H.

In: Journal of Biological Chemistry, Vol. 286, No. 41, 14.10.2011, p. 35823-35833.

Research output: Contribution to journalArticle

Meng, XW, Peterson, KL, Dai, H, Schneider, P, Lee, SH, Zhang, JS, Koenig, A, Bronk, S, Billadeau, DD, Gores, GJ & Kaufmann, SH 2011, 'High cell surface death receptor expression determines type I Versus type II signaling', Journal of Biological Chemistry, vol. 286, no. 41, pp. 35823-35833. https://doi.org/10.1074/jbc.M111.240432
Meng, Xue Wei ; Peterson, Kevin L. ; Dai, Haiming ; Schneider, Paula ; Lee, Sun Hee ; Zhang, Jin San ; Koenig, Alexander ; Bronk, Steve ; Billadeau, Daniel D ; Gores, Gregory James ; Kaufmann, Scott H. / High cell surface death receptor expression determines type I Versus type II signaling. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 41. pp. 35823-35833.
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