Hierarchy in the assembly of HLA-B27 and HLA-Cw3 molecules in transgenic mice

S. Pedrinaci, J. Hanson, C. David

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cell surface expression of human class I molecules in transgenic mice is dependent upon the available pool of β2-microglobulin (β2m) and the affinity between mouse β2m and human class I molecules. HLA-B27 and HLA-Cw3 transgenes can be expressed in mouse strains of the H-2 haplotypes b, f, k and x which encode two endogenous class I genes mapping to H-2K and H-2D. The human class I genes cannot be expressed on H-2(d) and H-2(q) haplotypes which encode three endogenous class I molecules (K,D,L). This suggests that there may be only enough mouse β2m molecules to support three class I molecules. When both the HLA-B27 and HLA-Cw3 genes are introduced into H-2b mice, only HLA-Cw3 reaches the cell surface. This suggests that HLA-Cw3 has a higher affinity than HLA-B27 for mouse β2m. The possible implications of our findings regarding the assembly, transport, and expression of class I MHC molecules in vivo are discussed.

Original languageEnglish (US)
Pages (from-to)130-137
Number of pages8
JournalImmunogenetics
Volume39
Issue number2
StatePublished - 1994

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HLA-B27 Antigen
Transgenic Mice
MHC Class I Genes
Haplotypes
Chromosome Mapping
Transgenes
HLA-C*03 antigen
Genes

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Pedrinaci, S., Hanson, J., & David, C. (1994). Hierarchy in the assembly of HLA-B27 and HLA-Cw3 molecules in transgenic mice. Immunogenetics, 39(2), 130-137.

Hierarchy in the assembly of HLA-B27 and HLA-Cw3 molecules in transgenic mice. / Pedrinaci, S.; Hanson, J.; David, C.

In: Immunogenetics, Vol. 39, No. 2, 1994, p. 130-137.

Research output: Contribution to journalArticle

Pedrinaci, S, Hanson, J & David, C 1994, 'Hierarchy in the assembly of HLA-B27 and HLA-Cw3 molecules in transgenic mice', Immunogenetics, vol. 39, no. 2, pp. 130-137.
Pedrinaci, S. ; Hanson, J. ; David, C. / Hierarchy in the assembly of HLA-B27 and HLA-Cw3 molecules in transgenic mice. In: Immunogenetics. 1994 ; Vol. 39, No. 2. pp. 130-137.
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