Hepatitis C virus NS5A protein protects against TNF-α mediated apoptotic cell death

Asish K. Ghosh, Mainak Majumder, Robert Steele, Keith Meyer, Ranjit Ray, Ratna B. Ray

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) often causes a prolonged and persistent infection which may lead to hepatocellular carcinoma. We have previously reported that the nonstructural 5A (NS5A) protein of HCV promotes cell growth [Ghosh, A.K., Steele, R., Meyer, K., Ray, R., Ray, R.B., 1999. Hepatitis C virus NS5A protein modulates cell cycle regulatory genes and promotes cell growth. J. Gen. Virol. 80, 1179-1183]. In this study, we investigated the role of HCV NS5A (genotype 1a, strain H) in TNF-α induced apoptotic cell death. HepG2 cells expressing NS5A exhibited an inhibitory role in relation to TNF-α mediated apoptotic cell death. The NS5A protein blocked the activation of caspase-3 and inhibited proteolytic cleavage of the death substrate poly (ADP-ribose) polymerase in TNF-α induced cells. Together, these results suggest that HCV NS5A protein protects against TNF-α mediated apoptotic cell death. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalVirus Research
Volume67
Issue number2
DOIs
StatePublished - Apr 2000
Externally publishedYes

Fingerprint

Cell Death
Hepacivirus
cdc Genes
Poly(ADP-ribose) Polymerases
Hep G2 Cells
Regulator Genes
Growth
Caspase 3
Hepatocellular Carcinoma
Genotype
Infection
hepatitis C virus NS-5 protein
Proteins

Keywords

  • Apoptosis
  • Hepatitis C virus
  • TNF-α

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Ghosh, A. K., Majumder, M., Steele, R., Meyer, K., Ray, R., & Ray, R. B. (2000). Hepatitis C virus NS5A protein protects against TNF-α mediated apoptotic cell death. Virus Research, 67(2), 173-178. https://doi.org/10.1016/S0168-1702(00)00141-6

Hepatitis C virus NS5A protein protects against TNF-α mediated apoptotic cell death. / Ghosh, Asish K.; Majumder, Mainak; Steele, Robert; Meyer, Keith; Ray, Ranjit; Ray, Ratna B.

In: Virus Research, Vol. 67, No. 2, 04.2000, p. 173-178.

Research output: Contribution to journalArticle

Ghosh, AK, Majumder, M, Steele, R, Meyer, K, Ray, R & Ray, RB 2000, 'Hepatitis C virus NS5A protein protects against TNF-α mediated apoptotic cell death', Virus Research, vol. 67, no. 2, pp. 173-178. https://doi.org/10.1016/S0168-1702(00)00141-6
Ghosh, Asish K. ; Majumder, Mainak ; Steele, Robert ; Meyer, Keith ; Ray, Ranjit ; Ray, Ratna B. / Hepatitis C virus NS5A protein protects against TNF-α mediated apoptotic cell death. In: Virus Research. 2000 ; Vol. 67, No. 2. pp. 173-178.
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