Hepatitis B DNA vaccine induces protective antibody responses in human non-responders to conventional vaccination

Scott T. Rottinghaus; Gregory A. Poland; Robert M. Jacobson; Lori J. Barr; Mike J. Roy

doi:10.1016/S0264-410X(03)00447-X

Hepatitis B DNA vaccine induces protective antibody responses in human non-responders to conventional vaccination

Scott T. Rottinghaus, Gregory A. Poland, Robert M. Jacobson, Lori J. Barr, Mike J. Roy

Research output: Contribution to journal › Article › peer-review

124 Scopus citations

Abstract

A novel DNA vaccine against hepatitis B virus was administered intraepidermally by particle-mediated epidermal delivery (PMED) to 16 human subjects who demonstrated absent or non-sustainable responses to conventional hepatitis B vaccination. Eleven subjects received three doses of vaccine at 56-day intervals, and five subjects received only a single vaccination. Each dose of vaccine contained 4μg of plasmid DNA encoding the hepatitis B surface antigen (HBsAg). The vaccine was safe and well tolerated. Remarkably, the DNA vaccine elicited antibody responses in 12 of the 16 subjects after a licensed subunit vaccine failed to induce a lasting response after ≥3 vaccinations. This study provides evidence in humans for protective immunogenicity of a particle-mediated DNA vaccine in subjects who have responded suboptimally to conventional vaccination.

Original language	English (US)
Pages (from-to)	4604-4608
Number of pages	5
Journal	Vaccine
Volume	21
Issue number	31
DOIs	https://doi.org/10.1016/S0264-410X(03)00447-X
State	Published - Nov 7 2003

Keywords

DNA vaccines
Hepatitis B
Immunogenicity
Particle-mediated delivery
Vaccination

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/S0264-410X(03)00447-X

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title = "Hepatitis B DNA vaccine induces protective antibody responses in human non-responders to conventional vaccination",

abstract = "A novel DNA vaccine against hepatitis B virus was administered intraepidermally by particle-mediated epidermal delivery (PMED) to 16 human subjects who demonstrated absent or non-sustainable responses to conventional hepatitis B vaccination. Eleven subjects received three doses of vaccine at 56-day intervals, and five subjects received only a single vaccination. Each dose of vaccine contained 4μg of plasmid DNA encoding the hepatitis B surface antigen (HBsAg). The vaccine was safe and well tolerated. Remarkably, the DNA vaccine elicited antibody responses in 12 of the 16 subjects after a licensed subunit vaccine failed to induce a lasting response after ≥3 vaccinations. This study provides evidence in humans for protective immunogenicity of a particle-mediated DNA vaccine in subjects who have responded suboptimally to conventional vaccination.",

keywords = "DNA vaccines, Hepatitis B, Immunogenicity, Particle-mediated delivery, Vaccination",

author = "Rottinghaus, {Scott T.} and Poland, {Gregory A.} and Jacobson, {Robert M.} and Barr, {Lori J.} and Roy, {Mike J.}",

note = "Funding Information: The authors thank the participants in this study and the study nurses, Shelly Roberts, Delana Weiss, and Mary Krystine. The authors also thank Kim S. Zabel, Norm Pinsky, and the Mayo Vaccine Research Group for assistance. This research was supported by PowderJect {\textregistered} Vaccines Inc.",

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doi = "10.1016/S0264-410X(03)00447-X",

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T1 - Hepatitis B DNA vaccine induces protective antibody responses in human non-responders to conventional vaccination

AU - Rottinghaus, Scott T.

AU - Poland, Gregory A.

AU - Jacobson, Robert M.

AU - Barr, Lori J.

AU - Roy, Mike J.

N1 - Funding Information: The authors thank the participants in this study and the study nurses, Shelly Roberts, Delana Weiss, and Mary Krystine. The authors also thank Kim S. Zabel, Norm Pinsky, and the Mayo Vaccine Research Group for assistance. This research was supported by PowderJect ® Vaccines Inc.

PY - 2003/11/7

Y1 - 2003/11/7

N2 - A novel DNA vaccine against hepatitis B virus was administered intraepidermally by particle-mediated epidermal delivery (PMED) to 16 human subjects who demonstrated absent or non-sustainable responses to conventional hepatitis B vaccination. Eleven subjects received three doses of vaccine at 56-day intervals, and five subjects received only a single vaccination. Each dose of vaccine contained 4μg of plasmid DNA encoding the hepatitis B surface antigen (HBsAg). The vaccine was safe and well tolerated. Remarkably, the DNA vaccine elicited antibody responses in 12 of the 16 subjects after a licensed subunit vaccine failed to induce a lasting response after ≥3 vaccinations. This study provides evidence in humans for protective immunogenicity of a particle-mediated DNA vaccine in subjects who have responded suboptimally to conventional vaccination.

AB - A novel DNA vaccine against hepatitis B virus was administered intraepidermally by particle-mediated epidermal delivery (PMED) to 16 human subjects who demonstrated absent or non-sustainable responses to conventional hepatitis B vaccination. Eleven subjects received three doses of vaccine at 56-day intervals, and five subjects received only a single vaccination. Each dose of vaccine contained 4μg of plasmid DNA encoding the hepatitis B surface antigen (HBsAg). The vaccine was safe and well tolerated. Remarkably, the DNA vaccine elicited antibody responses in 12 of the 16 subjects after a licensed subunit vaccine failed to induce a lasting response after ≥3 vaccinations. This study provides evidence in humans for protective immunogenicity of a particle-mediated DNA vaccine in subjects who have responded suboptimally to conventional vaccination.

KW - DNA vaccines

KW - Hepatitis B

KW - Immunogenicity

KW - Particle-mediated delivery

KW - Vaccination

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AN - SCOPUS:0142199964

SN - 0264-410X

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EP - 4608

JO - Vaccine

JF - Vaccine

IS - 31

ER -

Hepatitis B DNA vaccine induces protective antibody responses in human non-responders to conventional vaccination

Abstract

Keywords

ASJC Scopus subject areas

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