Hepatic utilization of exogenous nucleotide precursors for restoration of ATP after cold ischemia in rats

John D. Palombo, John L. Bowers, Melvin E. Clouse, Ann McCullough, R. Armour Forse, Bruce R. Bistrian

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The study objective was to assess hepatic utilization of exogenous adenosine or adenine to enhance ATP recovery in rat liver after cold ischemia. ATP was measured noninvasively by 31P nuclear magnetic resonance (NMR) in perfused livers before and after 18 h of cold ischemia. The hepatocellular concentration of ATP during the initial postischemic reperfusion without adenosine or adenine coinfusion was 60% of that in fresh liver. The ATP increased significantly (P < 0.001) to 139% and 82% of baseline in postischemic livers coinfused for 90 min with adenosine or adenine (final concentration, 1 mmol/L), respectively. Less than 0.5% of the excess adenosine was catabolized to uric acid. In conclusion, adenosine and, to a lesser extent, adenine are salvaged by liver after extended cold ischemia to enhance ATP restoration. Provision of these ATP precursors, as components of an enterai formulation may facilitate the repletion of liver ATP and foster early resumption of liver function after an ischemic insult.

Original languageEnglish (US)
Pages (from-to)420-427
Number of pages8
JournalAmerican Journal of Clinical Nutrition
Volume57
Issue number3
DOIs
StatePublished - Mar 1993

Keywords

  • ATP
  • Adenine
  • Adenosine
  • Ischemia
  • Liver
  • Nuclear magnetic resonance
  • Rats

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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