Heme oxygenase 2 is present in interstitial cell networks of the mouse small intestine

S. M. Miller, Gianrico Farrugia, P. F. Schmalz, L. G. Ermilov, M. D. Maines, J. H. Szurszewski

Research output: Contribution to journalArticle

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Abstract

Background and Aims: The interstitial cell (IC) network may be of fundamental importance in regulating gastrointestinal motility. Intestinal smooth muscle cells are depolarized in the absence of ICs, and there are no spontaneous slow waves. The messenger molecules between IC network and smooth muscle are unknown. Exogenous administration of CO relaxes the opossum internal anal sphincter and the guinea pig ileum, and it modulates potassium current and membrane potential of circular smooth muscle cells of the human jejunum. The aim of this study was to determine whether heme oxygenase (HO)- 1 and HO-2, enzymes that catalyze the production of CO, are present in the IC network of the mouse small intestine. Methods: Antibodies specific for c- Kit, HO-1, and HO-2 were used for immunohistochemistry. Confocal images were obtained and were volume rendered, and the images were converted into three- dimensional images. Results: HO-2-like but not HO-1-like immunoreactivity was found in IC networks associated with the myenteric plexus and the deep muscular plexus. Conclusions: HO-2 but not HO-1 is present in the IC cell network of the mouse small intestine. The enzymatic activity of HO-2 will result in the endogenous production of CO in IC networks of the mouse small intestine.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalGastroenterology
Volume114
Issue number2
DOIs
StatePublished - 1998

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Small Intestine
Heme Oxygenase-1
Carbon Monoxide
Smooth Muscle Myocytes
Opossums
Myenteric Plexus
Gastrointestinal Motility
Three-Dimensional Imaging
Anal Canal
Jejunum
Ileum
Membrane Potentials
heme oxygenase-2
Potassium
Guinea Pigs
Immunohistochemistry
Antibodies
Enzymes

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Miller, S. M., Farrugia, G., Schmalz, P. F., Ermilov, L. G., Maines, M. D., & Szurszewski, J. H. (1998). Heme oxygenase 2 is present in interstitial cell networks of the mouse small intestine. Gastroenterology, 114(2), 239-244. https://doi.org/10.1016/S0016-5085(98)70473-1

Heme oxygenase 2 is present in interstitial cell networks of the mouse small intestine. / Miller, S. M.; Farrugia, Gianrico; Schmalz, P. F.; Ermilov, L. G.; Maines, M. D.; Szurszewski, J. H.

In: Gastroenterology, Vol. 114, No. 2, 1998, p. 239-244.

Research output: Contribution to journalArticle

Miller, SM, Farrugia, G, Schmalz, PF, Ermilov, LG, Maines, MD & Szurszewski, JH 1998, 'Heme oxygenase 2 is present in interstitial cell networks of the mouse small intestine', Gastroenterology, vol. 114, no. 2, pp. 239-244. https://doi.org/10.1016/S0016-5085(98)70473-1
Miller, S. M. ; Farrugia, Gianrico ; Schmalz, P. F. ; Ermilov, L. G. ; Maines, M. D. ; Szurszewski, J. H. / Heme oxygenase 2 is present in interstitial cell networks of the mouse small intestine. In: Gastroenterology. 1998 ; Vol. 114, No. 2. pp. 239-244.
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