Hematopoietic stem cells from poor and good mobilizers are qualitatively equivalent

Liuyan Jiang, Sunny Malik, Mark R Litzow, Dennis Gastineau, Ivana Micallef, Vivek Roy, Lawrence Solberg, Abba Zubair

Research output: Contribution to journalArticle

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Abstract

BACKGROUND: Marrow damage from chemo- and radiation therapies has been suggested to affect quality and quantity of hematopoietic stem cell (HSC) products. We tested the hypothesis that CD34+ cells (HSCs) from low mobilizers are qualitatively inferior to HSCs from high mobilizers. STUDY DESIGN AND METHODS: HSC quality was defined by proportion of primitive HSC subsets (CD34+CD38-, CD34+HLA-DR-, and CD34+ in G0 stage of cell cycle), the proportion of HSCs that express CXCR4 and CD26 homing proteins, and days to neutrophil and platelet (PLT) engraftments after transplant. HSC content and CD34 subsets analyses were performed using flow cytometry following the ISHAGE protocol. We evaluated the HSC quantity and quality of 139 autologous filgrastim-mobilized HSC products. Patients were categorized into low, moderate, and high mobilizers if their total HSC collection was less than 3 × 10 6, 3 × 10 6 or more and less than 5 × 10 6, and 5 × 10 6/kg or more, respectively. RESULTS: The median number of primitive CD34 subsets increases with increasing HSC numbers and this association was significant (p = 0.001). However, when the ratios of the primitive CD34 subsets to total HSC counts were compared among the mobilization groups, the ratios were not significantly different. Coexpression of neither CD26 nor CXCR4 with CD34 antigen correlated with HSC mobilization. Evaluation of days to neutrophil engraftment among the mobilization groups did not show a significant difference (p = 0.1). However, days to PLT engraftment among the mobilization groups was significantly different (p = 0.05). CONCLUSION: The quality of HSCs from low mobilizers was comparable to HSCs from high mobilizers.

Original languageEnglish (US)
Pages (from-to)542-548
Number of pages7
JournalTransfusion
Volume52
Issue number3
DOIs
StatePublished - Mar 2012

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Hematopoietic Stem Cells
Neutrophils
Blood Platelets
Cell Count
CD34 Antigens
Hematopoietic Stem Cell Mobilization
HLA-DR Antigens
Cell Cycle
Flow Cytometry
Radiotherapy
Bone Marrow
Transplants

ASJC Scopus subject areas

  • Hematology
  • Immunology
  • Immunology and Allergy

Cite this

Hematopoietic stem cells from poor and good mobilizers are qualitatively equivalent. / Jiang, Liuyan; Malik, Sunny; Litzow, Mark R; Gastineau, Dennis; Micallef, Ivana; Roy, Vivek; Solberg, Lawrence; Zubair, Abba.

In: Transfusion, Vol. 52, No. 3, 03.2012, p. 542-548.

Research output: Contribution to journalArticle

Jiang, Liuyan ; Malik, Sunny ; Litzow, Mark R ; Gastineau, Dennis ; Micallef, Ivana ; Roy, Vivek ; Solberg, Lawrence ; Zubair, Abba. / Hematopoietic stem cells from poor and good mobilizers are qualitatively equivalent. In: Transfusion. 2012 ; Vol. 52, No. 3. pp. 542-548.
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