Abstract
Human renal cell carcinomas are characterized by an inflammatory infiltrate containing many T lymphocytes. Attempts to grow T cells from such tumors by culture in interleukin (IL)-2 have yielded heterogeneous populations of cells with functional characteristics typical of lymphokine-activated killer cells obtained by similar culture of cells from peripheral blood mononuclear cells. We examined a panel of surface markers expressed on T lymphocytes to determine if the CD4+T cells infiltrating human renal cell carcinomas are different from those in peripheral blood mononuclear cells. By flow cytometry analysis the CD4+T cells in a panel of freshly digested human renal cell carcinoma primary and metastatic tumors expressed the activation markers CD69 and HLA-DR and manifested an increase in CD45RO and a reciprocal decrease in CD45RA expression as compared with peripheral blood CD4+T cells. This suggests that CD4+T cells infiltrating renal cell carcinomas are activated and have encountered antigen. However, the expression of the IL-2R a chain (CD25) was not different in tumor-infiltrating CD4+T cells and peripheral blood CD4+T cells, suggesting that T cells infiltrating human renal cell carcinomas may have a block in proliferative capacity. The general failure of cultured tumor-infiltrating lymphocyte (TIL) from renal cell carcinoma to demonstrate tumor-specific reactivity may be due to the failure of such cells to grow in IL-2.
Original language | English (US) |
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Pages (from-to) | 39-46 |
Number of pages | 8 |
Journal | Journal of Immunotherapy |
Volume | 17 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1995 |
Keywords
- CD4T cells
- Phenotype
- Renal carcinoma cells
- Surface markers
- T lymphocytes.
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Pharmacology
- Cancer Research