Halothane reduces force and intracellular Ca2+ in airway smooth muscle independently of cyclic nucleotides

K. A. Jones, R. R. Lorenz, N. Morimoto, Gary C Sieck, David Oman Warner

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Halothane relaxes airway smooth muscle in part by a direct effect on the smooth muscle cell. The purpose of tiffs study was to investigate the possible role of cyclic nucleotides in this direct effect. Strips of canine tracheal smooth muscle in vitro were contracted with acetylcholine (ACh) and then exposed to 0.7-2.6% halothane. Isometric force and the intracellular concentrations of adenosine cyclic 3',5'-monophosphate ([cAMP](i)), guanosine cyclic 3',5'-monophosphate ([cGMP](i)), and free calcium ([Ca2+](i), using the fluorescent Ca2+-sensitive dye fura 2) were measured. ACh caused significant increases in force, [cAMP](i), [cGMP](i), and [Ca2+](i). Subsequent exposure of the strips to halothane caused an additional increase in [cAMP](i), decreases in force and [Ca2+](i), and no effect on [cGMP](i). The additional increase in [cAMP](i) was similar to that produced by a concentration of isoproterenol (0.03 μM) that caused equipotent relaxation. Indomethacin abolished the increase in [cAMP](i) produced by ACh and abolished the additional increase in [cAMP](i) produced by halothane. In contrast, indomethacin had no effect on the decreases in force and [Ca2+](i). These findings suggest that in canine tracheal smooth muscle contracted with ACh 1) halothane increases [cAMP](i) by a cyclooxygenase- dependent mechanism and 2) the increase in [cAMP](i) produced by halothane is not responsible for the relaxation or the decrease in [Ca2+](i).

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume268
Issue number2 12-2
StatePublished - 1995

Fingerprint

cyclic nucleotides
Cyclic Nucleotides
halothane
cyclic AMP
Halothane
Cyclic AMP
smooth muscle
Smooth Muscle
calcium
acetylcholine
Acetylcholine
indomethacin
Indomethacin
Canidae
cyclic GMP
dogs
prostaglandin synthase
adenosine
Cyclic GMP
myocytes

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine
  • Agricultural and Biological Sciences(all)

Cite this

Halothane reduces force and intracellular Ca2+ in airway smooth muscle independently of cyclic nucleotides. / Jones, K. A.; Lorenz, R. R.; Morimoto, N.; Sieck, Gary C; Warner, David Oman.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 268, No. 2 12-2, 1995.

Research output: Contribution to journalArticle

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