Halomonas sulfidaeris-dominated microbial community inhabits a 1.8 km-deep subsurface Cambrian sandstone reservoir

Yiran Dong, Charu Gupta Kumar, Nicholas Chia, Pan Jun Kim, Philip A. Miller, Nathan D. Price, Isaac K.O. Cann, Theodore M. Flynn, Robert A. Sanford, Ivan G. Krapac, Randall A. Locke, Pei Ying Hong, Hideyuki Tamaki, Wen Tso Liu, Roderick I. Mackie, Alvaro G. Hernandez, Chris L. Wright, Mark A. Mikel, Jared L. Walker, Mayandi SivaguruGlenn Fried, Anthony C. Yannarell, Bruce W. Fouke

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

A low-diversity microbial community, dominated by the γ-proteobacterium Halomonas sulfidaeris, was detected in samples of warm saline formation porewater collected from the Cambrian Mt. Simon Sandstone in the Illinois Basin of the North American Midcontinent (1.8 km/5872 ft burial depth, 50oC, pH 8, 181 bars pressure). These highly porous and permeable quartz arenite sandstones are directly analogous to reservoirs around the world targeted for largescale hydrocarbon extraction, as well as subsurface gas and carbon storage. A new downhole lowcontamination subsurface sampling probe was used to collect in situ formation water samples for microbial environmental metagenomic analyses. Multiple lines of evidence suggest that this H. sulfidaerisdominated subsurface microbial community is indigenous and not derived from drilling mud microbial contamination. Data to support this includes V1-V3 pyrosequencing of formation water and drilling mud, as well as comparison with previously published microbial analyses of drilling muds in other sites. Metabolic pathway reconstruction, constrained by thegeology, geochemistry and present-day environmental conditions of the Mt. Simon Sandstone, implies that H. sulfidaeris-dominated subsurface microbial community may utilize iron and nitrogen metabolisms and extensively recycle indigenous nutrients and substrates. The presence of aromatic compound metabolic pathways suggests this microbial community can readily adapt to and survive subsurface hydrocarbon migration.

Original languageEnglish (US)
Pages (from-to)1695-1708
Number of pages14
JournalEnvironmental Microbiology
Volume16
Issue number6
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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    Dong, Y., Kumar, C. G., Chia, N., Kim, P. J., Miller, P. A., Price, N. D., Cann, I. K. O., Flynn, T. M., Sanford, R. A., Krapac, I. G., Locke, R. A., Hong, P. Y., Tamaki, H., Liu, W. T., Mackie, R. I., Hernandez, A. G., Wright, C. L., Mikel, M. A., Walker, J. L., ... Fouke, B. W. (2014). Halomonas sulfidaeris-dominated microbial community inhabits a 1.8 km-deep subsurface Cambrian sandstone reservoir. Environmental Microbiology, 16(6), 1695-1708. https://doi.org/10.1111/1462-2920.12325