Halomonas sulfidaeris-dominated microbial community inhabits a 1.8 km-deep subsurface Cambrian sandstone reservoir

Yiran Dong, Charu Gupta Kumar, Nicholas D Chia, Pan Jun Kim, Philip A. Miller, Nathan D. Price, Isaac K O Cann, Theodore M. Flynn, Robert A. Sanford, Ivan G. Krapac, Randall A. Locke, Pei Ying Hong, Hideyuki Tamaki, Wen Tso Liu, Roderick I. Mackie, Alvaro G. Hernandez, Chris L. Wright, Mark A. Mikel, Jared L. Walker, Mayandi SivaguruGlenn Fried, Anthony C. Yannarell, Bruce W. Fouke

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A low-diversity microbial community, dominated by the γ-proteobacterium Halomonas sulfidaeris, was detected in samples of warm saline formation porewater collected from the Cambrian Mt. Simon Sandstone in the Illinois Basin of the North American Midcontinent (1.8 km/5872 ft burial depth, 50oC, pH 8, 181 bars pressure). These highly porous and permeable quartz arenite sandstones are directly analogous to reservoirs around the world targeted for largescale hydrocarbon extraction, as well as subsurface gas and carbon storage. A new downhole lowcontamination subsurface sampling probe was used to collect in situ formation water samples for microbial environmental metagenomic analyses. Multiple lines of evidence suggest that this H. sulfidaerisdominated subsurface microbial community is indigenous and not derived from drilling mud microbial contamination. Data to support this includes V1-V3 pyrosequencing of formation water and drilling mud, as well as comparison with previously published microbial analyses of drilling muds in other sites. Metabolic pathway reconstruction, constrained by thegeology, geochemistry and present-day environmental conditions of the Mt. Simon Sandstone, implies that H. sulfidaeris-dominated subsurface microbial community may utilize iron and nitrogen metabolisms and extensively recycle indigenous nutrients and substrates. The presence of aromatic compound metabolic pathways suggests this microbial community can readily adapt to and survive subsurface hydrocarbon migration.

Original languageEnglish (US)
Pages (from-to)1695-1708
Number of pages14
JournalEnvironmental Microbiology
Volume16
Issue number6
DOIs
StatePublished - 2014

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Halomonas
sandstone
Hydrocarbons
Metabolic Networks and Pathways
microbial communities
microbial community
drilling
Burial
Metagenomics
Proteobacteria
Quartz
mud
Water
formation water
biochemical pathways
hydrocarbons
Nitrogen
Carbon
Iron
Gases

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Halomonas sulfidaeris-dominated microbial community inhabits a 1.8 km-deep subsurface Cambrian sandstone reservoir. / Dong, Yiran; Kumar, Charu Gupta; Chia, Nicholas D; Kim, Pan Jun; Miller, Philip A.; Price, Nathan D.; Cann, Isaac K O; Flynn, Theodore M.; Sanford, Robert A.; Krapac, Ivan G.; Locke, Randall A.; Hong, Pei Ying; Tamaki, Hideyuki; Liu, Wen Tso; Mackie, Roderick I.; Hernandez, Alvaro G.; Wright, Chris L.; Mikel, Mark A.; Walker, Jared L.; Sivaguru, Mayandi; Fried, Glenn; Yannarell, Anthony C.; Fouke, Bruce W.

In: Environmental Microbiology, Vol. 16, No. 6, 2014, p. 1695-1708.

Research output: Contribution to journalArticle

Dong, Y, Kumar, CG, Chia, ND, Kim, PJ, Miller, PA, Price, ND, Cann, IKO, Flynn, TM, Sanford, RA, Krapac, IG, Locke, RA, Hong, PY, Tamaki, H, Liu, WT, Mackie, RI, Hernandez, AG, Wright, CL, Mikel, MA, Walker, JL, Sivaguru, M, Fried, G, Yannarell, AC & Fouke, BW 2014, 'Halomonas sulfidaeris-dominated microbial community inhabits a 1.8 km-deep subsurface Cambrian sandstone reservoir', Environmental Microbiology, vol. 16, no. 6, pp. 1695-1708. https://doi.org/10.1111/1462-2920.12325
Dong, Yiran ; Kumar, Charu Gupta ; Chia, Nicholas D ; Kim, Pan Jun ; Miller, Philip A. ; Price, Nathan D. ; Cann, Isaac K O ; Flynn, Theodore M. ; Sanford, Robert A. ; Krapac, Ivan G. ; Locke, Randall A. ; Hong, Pei Ying ; Tamaki, Hideyuki ; Liu, Wen Tso ; Mackie, Roderick I. ; Hernandez, Alvaro G. ; Wright, Chris L. ; Mikel, Mark A. ; Walker, Jared L. ; Sivaguru, Mayandi ; Fried, Glenn ; Yannarell, Anthony C. ; Fouke, Bruce W. / Halomonas sulfidaeris-dominated microbial community inhabits a 1.8 km-deep subsurface Cambrian sandstone reservoir. In: Environmental Microbiology. 2014 ; Vol. 16, No. 6. pp. 1695-1708.
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abstract = "A low-diversity microbial community, dominated by the γ-proteobacterium Halomonas sulfidaeris, was detected in samples of warm saline formation porewater collected from the Cambrian Mt. Simon Sandstone in the Illinois Basin of the North American Midcontinent (1.8 km/5872 ft burial depth, 50oC, pH 8, 181 bars pressure). These highly porous and permeable quartz arenite sandstones are directly analogous to reservoirs around the world targeted for largescale hydrocarbon extraction, as well as subsurface gas and carbon storage. A new downhole lowcontamination subsurface sampling probe was used to collect in situ formation water samples for microbial environmental metagenomic analyses. Multiple lines of evidence suggest that this H. sulfidaerisdominated subsurface microbial community is indigenous and not derived from drilling mud microbial contamination. Data to support this includes V1-V3 pyrosequencing of formation water and drilling mud, as well as comparison with previously published microbial analyses of drilling muds in other sites. Metabolic pathway reconstruction, constrained by thegeology, geochemistry and present-day environmental conditions of the Mt. Simon Sandstone, implies that H. sulfidaeris-dominated subsurface microbial community may utilize iron and nitrogen metabolisms and extensively recycle indigenous nutrients and substrates. The presence of aromatic compound metabolic pathways suggests this microbial community can readily adapt to and survive subsurface hydrocarbon migration.",
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T1 - Halomonas sulfidaeris-dominated microbial community inhabits a 1.8 km-deep subsurface Cambrian sandstone reservoir

AU - Dong, Yiran

AU - Kumar, Charu Gupta

AU - Chia, Nicholas D

AU - Kim, Pan Jun

AU - Miller, Philip A.

AU - Price, Nathan D.

AU - Cann, Isaac K O

AU - Flynn, Theodore M.

AU - Sanford, Robert A.

AU - Krapac, Ivan G.

AU - Locke, Randall A.

AU - Hong, Pei Ying

AU - Tamaki, Hideyuki

AU - Liu, Wen Tso

AU - Mackie, Roderick I.

AU - Hernandez, Alvaro G.

AU - Wright, Chris L.

AU - Mikel, Mark A.

AU - Walker, Jared L.

AU - Sivaguru, Mayandi

AU - Fried, Glenn

AU - Yannarell, Anthony C.

AU - Fouke, Bruce W.

PY - 2014

Y1 - 2014

N2 - A low-diversity microbial community, dominated by the γ-proteobacterium Halomonas sulfidaeris, was detected in samples of warm saline formation porewater collected from the Cambrian Mt. Simon Sandstone in the Illinois Basin of the North American Midcontinent (1.8 km/5872 ft burial depth, 50oC, pH 8, 181 bars pressure). These highly porous and permeable quartz arenite sandstones are directly analogous to reservoirs around the world targeted for largescale hydrocarbon extraction, as well as subsurface gas and carbon storage. A new downhole lowcontamination subsurface sampling probe was used to collect in situ formation water samples for microbial environmental metagenomic analyses. Multiple lines of evidence suggest that this H. sulfidaerisdominated subsurface microbial community is indigenous and not derived from drilling mud microbial contamination. Data to support this includes V1-V3 pyrosequencing of formation water and drilling mud, as well as comparison with previously published microbial analyses of drilling muds in other sites. Metabolic pathway reconstruction, constrained by thegeology, geochemistry and present-day environmental conditions of the Mt. Simon Sandstone, implies that H. sulfidaeris-dominated subsurface microbial community may utilize iron and nitrogen metabolisms and extensively recycle indigenous nutrients and substrates. The presence of aromatic compound metabolic pathways suggests this microbial community can readily adapt to and survive subsurface hydrocarbon migration.

AB - A low-diversity microbial community, dominated by the γ-proteobacterium Halomonas sulfidaeris, was detected in samples of warm saline formation porewater collected from the Cambrian Mt. Simon Sandstone in the Illinois Basin of the North American Midcontinent (1.8 km/5872 ft burial depth, 50oC, pH 8, 181 bars pressure). These highly porous and permeable quartz arenite sandstones are directly analogous to reservoirs around the world targeted for largescale hydrocarbon extraction, as well as subsurface gas and carbon storage. A new downhole lowcontamination subsurface sampling probe was used to collect in situ formation water samples for microbial environmental metagenomic analyses. Multiple lines of evidence suggest that this H. sulfidaerisdominated subsurface microbial community is indigenous and not derived from drilling mud microbial contamination. Data to support this includes V1-V3 pyrosequencing of formation water and drilling mud, as well as comparison with previously published microbial analyses of drilling muds in other sites. Metabolic pathway reconstruction, constrained by thegeology, geochemistry and present-day environmental conditions of the Mt. Simon Sandstone, implies that H. sulfidaeris-dominated subsurface microbial community may utilize iron and nitrogen metabolisms and extensively recycle indigenous nutrients and substrates. The presence of aromatic compound metabolic pathways suggests this microbial community can readily adapt to and survive subsurface hydrocarbon migration.

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