TY - JOUR
T1 - Glycosaminoglycan Content in Skin of the Tight‐Skin Mouse
AU - Ross, Stephen C.
AU - Osborn, Thomas G.
AU - Dorner, Robert W.
AU - Zuckner, Jack
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1983/5
Y1 - 1983/5
N2 - The tight‐skin (TSK) mouse has cutaneous changes similar to those found in the skin of patients with progressive systemic sclerosis (PSS). Previous studies have shown that both have common abnormalities in skin thickness, dry weight, and hydroxyproline content. In this study, glycosaminoglycans (GAGs), major components of the ground substance, were quantitated in skins from TSK mice and compared with age‐matched normal mice. Biochemical studies included determinations of hexosamines, uronic acids, and total GAGs by cetylpyridinium chloride precipitation. Dry weights and water‐fat content of skin biopsy specimens from TSK mice were also compared with those of normal mice. Hexosamine, uronic acid, total GAGs, and dry weight were increased in TSK mouse skin when compared with normal mouse skin. The water‐fat content did not differ significantly. These findings were similar to those known to occur in PSS skin, further suggesting that the TSK mouse might serve as an animal model for the skin changes found in PSS patients.
AB - The tight‐skin (TSK) mouse has cutaneous changes similar to those found in the skin of patients with progressive systemic sclerosis (PSS). Previous studies have shown that both have common abnormalities in skin thickness, dry weight, and hydroxyproline content. In this study, glycosaminoglycans (GAGs), major components of the ground substance, were quantitated in skins from TSK mice and compared with age‐matched normal mice. Biochemical studies included determinations of hexosamines, uronic acids, and total GAGs by cetylpyridinium chloride precipitation. Dry weights and water‐fat content of skin biopsy specimens from TSK mice were also compared with those of normal mice. Hexosamine, uronic acid, total GAGs, and dry weight were increased in TSK mouse skin when compared with normal mouse skin. The water‐fat content did not differ significantly. These findings were similar to those known to occur in PSS skin, further suggesting that the TSK mouse might serve as an animal model for the skin changes found in PSS patients.
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U2 - 10.1002/art.1780260512
DO - 10.1002/art.1780260512
M3 - Article
C2 - 6847727
AN - SCOPUS:0020522553
SN - 0004-3591
VL - 26
SP - 653
EP - 657
JO - Arthritis & Rheumatism
JF - Arthritis & Rheumatism
IS - 5
ER -