Giant eosinophil colonies from cultures of bone marrow cells

J. H. Butterfield, D. Weiler

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

To date, the small size and slow growth of eosinophil colonies in vitro has hampered study of cloned eosinophils. We found enhanced eosinophol colony size and numbers in methylcellulose cultures of bone marrow cells utilizing defined supplemented bovine calf serum (DSBCS) in combination with EL4 conditioned medium (EL4-CM). At days 9, 16 and 23 significantly more eosinophil colonies and more cells/colony were present in cultures incubated with DSBCS/EL4-CM than in cultures incubated with fetal calf serum/EL4-CM. The ability to generate large numbers of eosinophils in vitro should facilitate study of cloned eosinophils.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalIn Vitro
Volume22
Issue number3
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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