Abstract
To date, the small size and slow growth of eosinophil colonies in vitro has hampered study of cloned eosinophils. We found enhanced eosinophol colony size and numbers in methylcellulose cultures of bone marrow cells utilizing defined supplemented bovine calf serum (DSBCS) in combination with EL4 conditioned medium (EL4-CM). At days 9, 16 and 23 significantly more eosinophil colonies and more cells/colony were present in cultures incubated with DSBCS/EL4-CM than in cultures incubated with fetal calf serum/EL4-CM. The ability to generate large numbers of eosinophils in vitro should facilitate study of cloned eosinophils.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 157-163 |
| Number of pages | 7 |
| Journal | In Vitro |
| Volume | 22 |
| Issue number | 3 |
| State | Published - 1986 |
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ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Developmental Biology
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Giant eosinophil colonies from cultures of bone marrow cells. / Butterfield, J. H.; Weiler, D.
In: In Vitro, Vol. 22, No. 3, 1986, p. 157-163.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Giant eosinophil colonies from cultures of bone marrow cells
AU - Butterfield, J. H.
AU - Weiler, D.
PY - 1986
Y1 - 1986
N2 - To date, the small size and slow growth of eosinophil colonies in vitro has hampered study of cloned eosinophils. We found enhanced eosinophol colony size and numbers in methylcellulose cultures of bone marrow cells utilizing defined supplemented bovine calf serum (DSBCS) in combination with EL4 conditioned medium (EL4-CM). At days 9, 16 and 23 significantly more eosinophil colonies and more cells/colony were present in cultures incubated with DSBCS/EL4-CM than in cultures incubated with fetal calf serum/EL4-CM. The ability to generate large numbers of eosinophils in vitro should facilitate study of cloned eosinophils.
AB - To date, the small size and slow growth of eosinophil colonies in vitro has hampered study of cloned eosinophils. We found enhanced eosinophol colony size and numbers in methylcellulose cultures of bone marrow cells utilizing defined supplemented bovine calf serum (DSBCS) in combination with EL4 conditioned medium (EL4-CM). At days 9, 16 and 23 significantly more eosinophil colonies and more cells/colony were present in cultures incubated with DSBCS/EL4-CM than in cultures incubated with fetal calf serum/EL4-CM. The ability to generate large numbers of eosinophils in vitro should facilitate study of cloned eosinophils.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022637162&partnerID=8YFLogxK
M3 - Article
C2 - 3949679
AN - SCOPUS:0022637162
VL - 22
SP - 157
EP - 163
JO - In Vitro
JF - In Vitro
SN - 0073-5655
IS - 3
ER -