Germ-free (GF) mice are a relevant model system to study host–microbial interactions in health and disease. In this chapter, we underscore the importance of using GF mice model to study host–microbial interactions in obesity, immune development and gastrointestinal physiology by reviewing current literature. Furthermore, we also provide a brief protocol on how to setup a gnotobiotic facility in order to properly maintain and assess GF status in mice colonies.
|Original language||English (US)|
|Number of pages||13|
|Journal||Methods in Molecular Biology|
|State||Published - May 1 2016|
- Gut microbiome
ASJC Scopus subject areas
- Molecular Biology