Genomic organization and mutation analysis of p73 in oligodendrogliomas with chromosome 1 p-arm deletions

Ming Mai, Haojie Huang, Christopher Reed, Chiping Qian, Justin S. Smith, Benjamin Alderete, Robert Brian Jenkins, David I Smith, Wanguo Liu

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

p73, a protein having substantial structural and functional similarity to p53, has recently been identified and demonstrated to be a potential tumor suppressor. Its location on human chromosome 1p36.33 implicates p73 as a candidate for neuroblastoma. Like neuroblastoma, oligodendrogliomas also show a high frequency of deletions in chromosome 1p36.3. To determine whether p73 is a potential tumor suppressor gene involved in the development of oligodendrogliomas, we performed mutation analysis of p73 in oligodendrogliomas with chromosome 1 p-arm deletions. We first determined the genomic organization and the intron-exon boundary sequences of the p73 gene by long PCR, vectorette PCR, and Southern hybridization. This gene spans about 65 kb with a large first intron. Primer pairs for the amplification of each of the 13 p73 encoding exons were designed in corresponding introns. The amplicons were then analyzed using the denaturing high-performance liquid chromatography system for mutations in the p73 gene. Twenty oligodendroglioma samples with 1p36.3 deletions were screened, but no mutations were detected except for several polymorphisms. It is thus clear that p73 is not a candidate gene for oligodendroglioma despite its location in the frequently deleted 1p36.3 region.

Original languageEnglish (US)
Pages (from-to)359-363
Number of pages5
JournalGenomics
Volume51
Issue number3
DOIs
StatePublished - Aug 1 1998

Fingerprint

Oligodendroglioma
Chromosomes, Human, Pair 1
Mutation
Introns
Neuroblastoma
Genes
Exons
Polymerase Chain Reaction
Chromosome Deletion
Human Chromosomes
Tumor Suppressor Genes
High Pressure Liquid Chromatography
Neoplasms

ASJC Scopus subject areas

  • Genetics

Cite this

Genomic organization and mutation analysis of p73 in oligodendrogliomas with chromosome 1 p-arm deletions. / Mai, Ming; Huang, Haojie; Reed, Christopher; Qian, Chiping; Smith, Justin S.; Alderete, Benjamin; Jenkins, Robert Brian; Smith, David I; Liu, Wanguo.

In: Genomics, Vol. 51, No. 3, 01.08.1998, p. 359-363.

Research output: Contribution to journalArticle

Mai, Ming ; Huang, Haojie ; Reed, Christopher ; Qian, Chiping ; Smith, Justin S. ; Alderete, Benjamin ; Jenkins, Robert Brian ; Smith, David I ; Liu, Wanguo. / Genomic organization and mutation analysis of p73 in oligodendrogliomas with chromosome 1 p-arm deletions. In: Genomics. 1998 ; Vol. 51, No. 3. pp. 359-363.
@article{f872638a801e49739b8a494dcfd21048,
title = "Genomic organization and mutation analysis of p73 in oligodendrogliomas with chromosome 1 p-arm deletions",
abstract = "p73, a protein having substantial structural and functional similarity to p53, has recently been identified and demonstrated to be a potential tumor suppressor. Its location on human chromosome 1p36.33 implicates p73 as a candidate for neuroblastoma. Like neuroblastoma, oligodendrogliomas also show a high frequency of deletions in chromosome 1p36.3. To determine whether p73 is a potential tumor suppressor gene involved in the development of oligodendrogliomas, we performed mutation analysis of p73 in oligodendrogliomas with chromosome 1 p-arm deletions. We first determined the genomic organization and the intron-exon boundary sequences of the p73 gene by long PCR, vectorette PCR, and Southern hybridization. This gene spans about 65 kb with a large first intron. Primer pairs for the amplification of each of the 13 p73 encoding exons were designed in corresponding introns. The amplicons were then analyzed using the denaturing high-performance liquid chromatography system for mutations in the p73 gene. Twenty oligodendroglioma samples with 1p36.3 deletions were screened, but no mutations were detected except for several polymorphisms. It is thus clear that p73 is not a candidate gene for oligodendroglioma despite its location in the frequently deleted 1p36.3 region.",
author = "Ming Mai and Haojie Huang and Christopher Reed and Chiping Qian and Smith, {Justin S.} and Benjamin Alderete and Jenkins, {Robert Brian} and Smith, {David I} and Wanguo Liu",
year = "1998",
month = "8",
day = "1",
doi = "10.1006/geno.1998.5387",
language = "English (US)",
volume = "51",
pages = "359--363",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Genomic organization and mutation analysis of p73 in oligodendrogliomas with chromosome 1 p-arm deletions

AU - Mai, Ming

AU - Huang, Haojie

AU - Reed, Christopher

AU - Qian, Chiping

AU - Smith, Justin S.

AU - Alderete, Benjamin

AU - Jenkins, Robert Brian

AU - Smith, David I

AU - Liu, Wanguo

PY - 1998/8/1

Y1 - 1998/8/1

N2 - p73, a protein having substantial structural and functional similarity to p53, has recently been identified and demonstrated to be a potential tumor suppressor. Its location on human chromosome 1p36.33 implicates p73 as a candidate for neuroblastoma. Like neuroblastoma, oligodendrogliomas also show a high frequency of deletions in chromosome 1p36.3. To determine whether p73 is a potential tumor suppressor gene involved in the development of oligodendrogliomas, we performed mutation analysis of p73 in oligodendrogliomas with chromosome 1 p-arm deletions. We first determined the genomic organization and the intron-exon boundary sequences of the p73 gene by long PCR, vectorette PCR, and Southern hybridization. This gene spans about 65 kb with a large first intron. Primer pairs for the amplification of each of the 13 p73 encoding exons were designed in corresponding introns. The amplicons were then analyzed using the denaturing high-performance liquid chromatography system for mutations in the p73 gene. Twenty oligodendroglioma samples with 1p36.3 deletions were screened, but no mutations were detected except for several polymorphisms. It is thus clear that p73 is not a candidate gene for oligodendroglioma despite its location in the frequently deleted 1p36.3 region.

AB - p73, a protein having substantial structural and functional similarity to p53, has recently been identified and demonstrated to be a potential tumor suppressor. Its location on human chromosome 1p36.33 implicates p73 as a candidate for neuroblastoma. Like neuroblastoma, oligodendrogliomas also show a high frequency of deletions in chromosome 1p36.3. To determine whether p73 is a potential tumor suppressor gene involved in the development of oligodendrogliomas, we performed mutation analysis of p73 in oligodendrogliomas with chromosome 1 p-arm deletions. We first determined the genomic organization and the intron-exon boundary sequences of the p73 gene by long PCR, vectorette PCR, and Southern hybridization. This gene spans about 65 kb with a large first intron. Primer pairs for the amplification of each of the 13 p73 encoding exons were designed in corresponding introns. The amplicons were then analyzed using the denaturing high-performance liquid chromatography system for mutations in the p73 gene. Twenty oligodendroglioma samples with 1p36.3 deletions were screened, but no mutations were detected except for several polymorphisms. It is thus clear that p73 is not a candidate gene for oligodendroglioma despite its location in the frequently deleted 1p36.3 region.

UR - http://www.scopus.com/inward/record.url?scp=0032143910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032143910&partnerID=8YFLogxK

U2 - 10.1006/geno.1998.5387

DO - 10.1006/geno.1998.5387

M3 - Article

VL - 51

SP - 359

EP - 363

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -