TY - JOUR
T1 - Genomic analysis and selected molecular pathways in rare cancers
AU - Liu, Stephen V.
AU - Lenkiewicz, Elizabeth
AU - Evers, Lisa
AU - Holley, Tara
AU - Kiefer, Jeffrey
AU - Ruiz, Christian
AU - Glatz, Katharina
AU - Bubendorf, Lukas
AU - Demeure, Michael J.
AU - Eng, Cathy
AU - Ramanathan, Ramesh K.
AU - Von Hoff, Daniel D.
AU - Barrett, Michael T.
PY - 2012/12
Y1 - 2012/12
N2 - It is widely accepted that many cancers arise as a result of an acquired genomic instability and the subsequent evolution of tumor cells with variable patterns of selected and background aberrations. The presence and behaviors of distinct neoplastic cell populations within a patient's tumor may underlie multiple clinical phenotypes in cancers. A goal of many current cancer genome studies is the identification of recurring selected driver events that can be advanced for the development of personalized therapies. Unfortunately, in the majority of rare tumors, this type of analysis can be particularly challenging. Large series of specimens for analysis are simply not available, allowing recurring patterns to remain hidden. In this paper, we highlight the use of DNA content-based flow sorting to identify and isolate DNA-diploid and DNA-aneuploid populations from tumor biopsies as a strategy to comprehensively study the genomic composition and behaviors of individual cancers in a series of rare solid tumors: intrahepatic cholangiocarcinoma, anal carcinoma, adrenal leiomyosarcoma, and pancreatic neuroendocrine tumors. We propose that the identification of highly selected genomic events in distinct tumor populations within each tumor can identify candidate driver events that can facilitate the development of novel, personalized treatment strategies for patients with cancer.
AB - It is widely accepted that many cancers arise as a result of an acquired genomic instability and the subsequent evolution of tumor cells with variable patterns of selected and background aberrations. The presence and behaviors of distinct neoplastic cell populations within a patient's tumor may underlie multiple clinical phenotypes in cancers. A goal of many current cancer genome studies is the identification of recurring selected driver events that can be advanced for the development of personalized therapies. Unfortunately, in the majority of rare tumors, this type of analysis can be particularly challenging. Large series of specimens for analysis are simply not available, allowing recurring patterns to remain hidden. In this paper, we highlight the use of DNA content-based flow sorting to identify and isolate DNA-diploid and DNA-aneuploid populations from tumor biopsies as a strategy to comprehensively study the genomic composition and behaviors of individual cancers in a series of rare solid tumors: intrahepatic cholangiocarcinoma, anal carcinoma, adrenal leiomyosarcoma, and pancreatic neuroendocrine tumors. We propose that the identification of highly selected genomic events in distinct tumor populations within each tumor can identify candidate driver events that can facilitate the development of novel, personalized treatment strategies for patients with cancer.
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U2 - 10.1088/1478-3975/9/6/065004
DO - 10.1088/1478-3975/9/6/065004
M3 - Article
C2 - 23196986
AN - SCOPUS:84871244101
SN - 1478-3967
VL - 9
JO - Physical Biology
JF - Physical Biology
IS - 6
M1 - 065004
ER -