Genetic variability of the murine creatine kinase B gene locus and related pseudogenes in different inbred strains of mice

Jan Van Deursen, Jan Schepens, Wilma Peters, Dies Meijer, Gerard Grosveld, Wiljan Hendriks, Bé Wieringa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The role of genetic variation in isoenzyme gene families is often poorly appreciated. We report here on the determination of DNA sequences and typing of genetic variability in four creatine kinase B (CKB) gene loci in different inbred strains of mice. The unique functional murine CKB gene was found to be nearly identical to the previously characterised rat and human sequences in both size and exon-intron structure. In this gene, approximately 0.5% allelic nucleotide positions as well as the lengths of simple A-rich and [TG]n repetitive elements located at the 5′ and 3′ sides of the transcribed segment, differed between inbred strains of mice. Preliminary experiments suggest that this sequence divergence is of importance for design of gene targeting strategies involving homologous DNA recombination. The three additional CKB-like gene loci in mice all had the characteristics of processed pseudogenes. By Southern blot analysis we could demonstrate that both the type and number of pseudogenes differed between inbred strains. Analysis of the CKB gene sequences enabled us to speculate about the evolutionary history of this highly polymorphic subfamily of genes.

Original languageEnglish (US)
Pages (from-to)340-349
Number of pages10
JournalGenomics
Volume12
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Pseudogenes
Inbred Strains Mice
Creatine Kinase
Genes
DNA Fingerprinting
Gene Targeting
Homologous Recombination
Southern Blotting
Introns
Isoenzymes
Exons
Nucleotides
History
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Genetic variability of the murine creatine kinase B gene locus and related pseudogenes in different inbred strains of mice. / Van Deursen, Jan; Schepens, Jan; Peters, Wilma; Meijer, Dies; Grosveld, Gerard; Hendriks, Wiljan; Wieringa, Bé.

In: Genomics, Vol. 12, No. 2, 1992, p. 340-349.

Research output: Contribution to journalArticle

Van Deursen, Jan ; Schepens, Jan ; Peters, Wilma ; Meijer, Dies ; Grosveld, Gerard ; Hendriks, Wiljan ; Wieringa, Bé. / Genetic variability of the murine creatine kinase B gene locus and related pseudogenes in different inbred strains of mice. In: Genomics. 1992 ; Vol. 12, No. 2. pp. 340-349.
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