Genetic expression of endothelial nitric oxide synthase in human atrial myocardium

Chi Ming Wei, Shi Wen Jiang, John A. Lust, Richard C. Daly, Christopher G A Mcgregor

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objective: To investigate the expression of endothelial nitric oxide synthase (eNOS) in human cardiac tissues. Design: We attempted to determine the genetic expression and localization of eNOS in normal human atrial tissue. Material and Methods: In normal human right atrial tissues from five donors during cardiac transplantation, eNOS expression and localization were assessed by using Northern blot analysis, in situ hybridization, and immunohistochemical staining. Results: Northern blot analysis and in situ hybridization demonstrated that eNOS messenger RNA is present in cardiomyocytes. Positive immunohistochemical staining was observed in the cytoplasm of cardiomyocytes. Conclusion: These studies show for the first time the genetic expression and distribution of eNOS in human atrial myocardium and suggest that the eNOS-mediated paracrine and autocrine pathway may participate in the control of myocardial function in humans.

Original languageEnglish (US)
Pages (from-to)346-350
Number of pages5
JournalMayo Clinic Proceedings
Volume71
Issue number4
StatePublished - 1996

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Nitric Oxide Synthase Type III
Myocardium
Cardiac Myocytes
Northern Blotting
In Situ Hybridization
Staining and Labeling
Heart Transplantation
Cytoplasm
Tissue Donors
Messenger RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Wei, C. M., Jiang, S. W., Lust, J. A., Daly, R. C., & Mcgregor, C. G. A. (1996). Genetic expression of endothelial nitric oxide synthase in human atrial myocardium. Mayo Clinic Proceedings, 71(4), 346-350.

Genetic expression of endothelial nitric oxide synthase in human atrial myocardium. / Wei, Chi Ming; Jiang, Shi Wen; Lust, John A.; Daly, Richard C.; Mcgregor, Christopher G A.

In: Mayo Clinic Proceedings, Vol. 71, No. 4, 1996, p. 346-350.

Research output: Contribution to journalArticle

Wei, CM, Jiang, SW, Lust, JA, Daly, RC & Mcgregor, CGA 1996, 'Genetic expression of endothelial nitric oxide synthase in human atrial myocardium', Mayo Clinic Proceedings, vol. 71, no. 4, pp. 346-350.
Wei CM, Jiang SW, Lust JA, Daly RC, Mcgregor CGA. Genetic expression of endothelial nitric oxide synthase in human atrial myocardium. Mayo Clinic Proceedings. 1996;71(4):346-350.
Wei, Chi Ming ; Jiang, Shi Wen ; Lust, John A. ; Daly, Richard C. ; Mcgregor, Christopher G A. / Genetic expression of endothelial nitric oxide synthase in human atrial myocardium. In: Mayo Clinic Proceedings. 1996 ; Vol. 71, No. 4. pp. 346-350.
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