Genetic expression of endothelial nitric oxide synthase in human atrial myocardium

Chi Ming Wei, Shi Wen Jiang, John A. Lust, Richard C. Daly, Christopher G.A. Mcgregor

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Objective: To investigate the expression of endothelial nitric oxide synthase (eNOS) in human cardiac tissues. Design: We attempted to determine the genetic expression and localization of eNOS in normal human atrial tissue. Material and Methods: In normal human right atrial tissues from five donors during cardiac transplantation, eNOS expression and localization were assessed by using Northern blot analysis, in situ hybridization, and immunohistochemical staining. Results: Northern blot analysis and in situ hybridization demonstrated that eNOS messenger RNA is present in cardiomyocytes. Positive immunohistochemical staining was observed in the cytoplasm of cardiomyocytes. Conclusion: These studies show for the first time the genetic expression and distribution of eNOS in human atrial myocardium and suggest that the eNOS-mediated paracrine and autocrine pathway may participate in the control of myocardial function in humans.

Original languageEnglish (US)
Pages (from-to)346-350
Number of pages5
JournalMayo Clinic proceedings
Volume71
Issue number4
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Medicine(all)

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    Wei, C. M., Jiang, S. W., Lust, J. A., Daly, R. C., & Mcgregor, C. G. A. (1996). Genetic expression of endothelial nitric oxide synthase in human atrial myocardium. Mayo Clinic proceedings, 71(4), 346-350. https://doi.org/10.4065/71.4.346