Generation and phenotypic characterization of Pde1a mutant mice

Xiaofang Wang, Satsuki Yamada, Wells B. Lariviere, Hong Ye, Jason L. Bakeberg, Maria Irazabal Mira, Fouad Chebib, Jan Van Deursen, Peter C Harris, Caroline R. Sussman, Atta Behfar, Christopher J. Ward, Vicente Torres

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

It has been proposed that a reduction in intracellular calcium causes an increase in intracellular cAMP and PKA activity through stimulation of calcium inhibitable adenylyl cyclase 6 and inhibition of phosphodiesterase 1 (PDE1), the main enzymes generating and degrading cAMP in the distal nephron and collecting duct, thus contributing to the development and progression of autosomal dominant polycystic kidney disease (ADPKD). In zebrafish pde1a depletion aggravates and overexpression ameliorates the cystic phenotype. To study the role of PDE1A in a mammalian system, we used a TALEN pair to Pde1a exon 7, targeting the histidine-aspartic acid dipeptide involved in ligating the active site Zn++ ion to generate two Pde1a null mouse lines. Pde1a mutants had a mild renal cystic disease and a urine concentrating defect (associated with upregulation of PDE4 activity and decreased protein kinase A dependent phosphorylation of aquaporin-2) on a wild-type genetic background and aggravated renal cystic disease on a Pkd2WS25/- background. Pde1a mutants additionally had lower aortic blood pressure and increased left ventricular (LV) ejection fraction, without a change in LV mass index, consistent with the high aortic and low cardiac expression of Pde1a in wild-type mice. These results support an important role of PDE1A in the renal pathogenesis of ADPKD and in the regulation of blood pressure.

Original languageEnglish (US)
Article numbere0181087
JournalPLoS One
Volume12
Issue number7
DOIs
StatePublished - Jul 1 2017

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Cystic Kidney Diseases
Autosomal Dominant Polycystic Kidney
kidneys
Aquaporin 2
Calcium
mutants
blood pressure
Dipeptides
Nephrons
Blood pressure
mice
Phosphoric Diester Hydrolases
Zebrafish
Cyclic AMP-Dependent Protein Kinases
Histidine
Aspartic Acid
Stroke Volume
calcium
Exons
nephrons

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Generation and phenotypic characterization of Pde1a mutant mice. / Wang, Xiaofang; Yamada, Satsuki; Lariviere, Wells B.; Ye, Hong; Bakeberg, Jason L.; Irazabal Mira, Maria; Chebib, Fouad; Van Deursen, Jan; Harris, Peter C; Sussman, Caroline R.; Behfar, Atta; Ward, Christopher J.; Torres, Vicente.

In: PLoS One, Vol. 12, No. 7, e0181087, 01.07.2017.

Research output: Contribution to journalArticle

Wang, Xiaofang ; Yamada, Satsuki ; Lariviere, Wells B. ; Ye, Hong ; Bakeberg, Jason L. ; Irazabal Mira, Maria ; Chebib, Fouad ; Van Deursen, Jan ; Harris, Peter C ; Sussman, Caroline R. ; Behfar, Atta ; Ward, Christopher J. ; Torres, Vicente. / Generation and phenotypic characterization of Pde1a mutant mice. In: PLoS One. 2017 ; Vol. 12, No. 7.
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