TY - JOUR
T1 - Generation and characterization of monoclonal antibodies to NIAM
T2 - A nuclear interactor of ARF and Mdm2
AU - Hagen, Jussara
AU - Tompkins, Van
AU - Dudakovic, Amel
AU - Weydert, Jamie A.
AU - Quelle, Dawn E.
PY - 2008/6/1
Y1 - 2008/6/1
N2 - Nuclear interactor of ARF and Mdm2 (NIAM) is a newly discovered growth inhibitor that helps maintain chromosomal stability. It is functionally linked to the ARF-Mdm2-p53 tumor suppressor pathway and is predicted to be a tumor suppressor, but the lack of antibodies capable of detecting the endogenous human protein has delayed efforts to define its role in human tumorigenesis. This study reports the development, screening, and characterization of several monoclonal antibodies (MAbs) that specifically recognize endogenous human NIAM protein by Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. These MAbs are predicted to be important tools for evaluating the expression and physiological function of NIAM in normal versus neoplastic human cells and tissues.
AB - Nuclear interactor of ARF and Mdm2 (NIAM) is a newly discovered growth inhibitor that helps maintain chromosomal stability. It is functionally linked to the ARF-Mdm2-p53 tumor suppressor pathway and is predicted to be a tumor suppressor, but the lack of antibodies capable of detecting the endogenous human protein has delayed efforts to define its role in human tumorigenesis. This study reports the development, screening, and characterization of several monoclonal antibodies (MAbs) that specifically recognize endogenous human NIAM protein by Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. These MAbs are predicted to be important tools for evaluating the expression and physiological function of NIAM in normal versus neoplastic human cells and tissues.
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U2 - 10.1089/hyb.2007.0533
DO - 10.1089/hyb.2007.0533
M3 - Article
C2 - 18582208
AN - SCOPUS:50349092050
SN - 1554-0014
VL - 27
SP - 159
EP - 166
JO - Hybridoma
JF - Hybridoma
IS - 3
ER -