Gene transcription changes in asthmatic chronic rhinosinusitis with nasal polyps and comparison to those in atopic dermatitis

Douglas A. Plager, Jane C. Kahl, Yan Asmann, Allan E. Nilson, John F. Pallanch, Oren Friedman, Hirohito Kita

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: Asthmatic chronic rhinosinusitis with nasal polyps (aCRSwNP) is a common disruptive eosinophilic disease without effective medical treatment. Therefore, we sought to identify gene expression changes, particularly those occurring early, in aCRSwNP. To highlight expression changes associated with eosinophilic epithelial inflammation, we further compared the changes in aCRSwNP with those in a second eosinophilic epithelial disease, atopic dermatitis (AD), which is also closely related to asthma. Methods/Principal Findings: Genome-wide mRNA levels measured by exon array in both nasosinus inflamed mucosa and adjacent polyp from 11 aCRSwNP patients were compared to those in nasosinus tissue from 17 normal or rhinitis subjects without polyps. Differential expression of selected genes was confirmed by qRT-PCR or immunoassay, and transcription changes common to AD were identified. Comparison of aCRSwNP inflamed mucosa and polyp to normal/rhinitis tissue identified 447 differentially transcribed genes at ≥2 fold-change and adjusted p-value<0.05. These included increased transcription of chemokines localized to chromosome 17q11.2 (CCL13, CCL2, CCL8, and CCL11) that favor eosinophil and monocyte chemotaxis and chemokines (CCL18, CCL22, and CXCL13) that alternatively-activated monocyte-derived cells have been shown to produce. Additional transcription changes likely associated with Th2-like eosinophilic inflammation were prominent and included increased IL1RL1 (IL33 receptor) and EMR1&3 and decreased CRISP2&3. A down-regulated PDGFB-centric network involving several smooth muscle-associated genes was also implicated. Genes at 17q11.2, genes associated with alternative activation or smooth muscle, and the IL1RL1 gene were also differentially transcribed in AD. Conclusions/Significance: Our data implicate several genes or gene sets in aCRSwNP and eosinophilic epithelial inflammation, some that likely act in the earlier stages of inflammation. The identified gene expression changes provide additional diagnostic and therapeutic targets for aCRSwNP and other eosinophilic epithelial diseases.

Original languageEnglish (US)
Article numbere11450
JournalPLoS One
Volume5
Issue number7
DOIs
StatePublished - 2010

Fingerprint

Nasal Polyps
atopic dermatitis
Atopic Dermatitis
Transcription
transcription (genetics)
Genes
Polyps
inflammation
genes
Inflammation
rhinitis
Rhinitis
Gene Expression
monocytes
smooth muscle
gene expression
mucosa
Smooth Muscle
Chemokine CXCL13
Monocytes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Gene transcription changes in asthmatic chronic rhinosinusitis with nasal polyps and comparison to those in atopic dermatitis. / Plager, Douglas A.; Kahl, Jane C.; Asmann, Yan; Nilson, Allan E.; Pallanch, John F.; Friedman, Oren; Kita, Hirohito.

In: PLoS One, Vol. 5, No. 7, e11450, 2010.

Research output: Contribution to journalArticle

Plager, Douglas A. ; Kahl, Jane C. ; Asmann, Yan ; Nilson, Allan E. ; Pallanch, John F. ; Friedman, Oren ; Kita, Hirohito. / Gene transcription changes in asthmatic chronic rhinosinusitis with nasal polyps and comparison to those in atopic dermatitis. In: PLoS One. 2010 ; Vol. 5, No. 7.
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