Gene identification by arrested primer extension

G. S. Sandhu, B. C. Kline, M. E. Bolander, G. Sarkar

Research output: Contribution to journalArticle

Abstract

This paper reports a novel method for the identification of nucleic acid target sequences when these targets have high sequence identity. Homologous genes are currently identified by sequencing. We hypothesize that by primer extension in the presence of selected nucleotides, genes with similar sequence can be identified by the length of the extension products on gel electrophoresis. This simple procedure eliminates the much-demanding process of sequencing. We term this process Arrested Primer Extension (APE). As a demonstration of the feasibility of this method, we have used APE to speciate a known set of cultured mycobacteria. There should be many other applications of this method.

Original languageEnglish (US)
Pages (from-to)951-955
Number of pages5
JournalBioTechniques
Volume14
Issue number6
StatePublished - 1993

Fingerprint

Genes
Electrophoresis
Nucleic Acids
Demonstrations
Nucleotides
Gels
Mycobacterium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

Cite this

Sandhu, G. S., Kline, B. C., Bolander, M. E., & Sarkar, G. (1993). Gene identification by arrested primer extension. BioTechniques, 14(6), 951-955.

Gene identification by arrested primer extension. / Sandhu, G. S.; Kline, B. C.; Bolander, M. E.; Sarkar, G.

In: BioTechniques, Vol. 14, No. 6, 1993, p. 951-955.

Research output: Contribution to journalArticle

Sandhu, GS, Kline, BC, Bolander, ME & Sarkar, G 1993, 'Gene identification by arrested primer extension', BioTechniques, vol. 14, no. 6, pp. 951-955.
Sandhu GS, Kline BC, Bolander ME, Sarkar G. Gene identification by arrested primer extension. BioTechniques. 1993;14(6):951-955.
Sandhu, G. S. ; Kline, B. C. ; Bolander, M. E. ; Sarkar, G. / Gene identification by arrested primer extension. In: BioTechniques. 1993 ; Vol. 14, No. 6. pp. 951-955.
@article{9ef9468168b04976998673217e725644,
title = "Gene identification by arrested primer extension",
abstract = "This paper reports a novel method for the identification of nucleic acid target sequences when these targets have high sequence identity. Homologous genes are currently identified by sequencing. We hypothesize that by primer extension in the presence of selected nucleotides, genes with similar sequence can be identified by the length of the extension products on gel electrophoresis. This simple procedure eliminates the much-demanding process of sequencing. We term this process Arrested Primer Extension (APE). As a demonstration of the feasibility of this method, we have used APE to speciate a known set of cultured mycobacteria. There should be many other applications of this method.",
author = "Sandhu, {G. S.} and Kline, {B. C.} and Bolander, {M. E.} and G. Sarkar",
year = "1993",
language = "English (US)",
volume = "14",
pages = "951--955",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Eaton Publishing Company",
number = "6",

}

TY - JOUR

T1 - Gene identification by arrested primer extension

AU - Sandhu, G. S.

AU - Kline, B. C.

AU - Bolander, M. E.

AU - Sarkar, G.

PY - 1993

Y1 - 1993

N2 - This paper reports a novel method for the identification of nucleic acid target sequences when these targets have high sequence identity. Homologous genes are currently identified by sequencing. We hypothesize that by primer extension in the presence of selected nucleotides, genes with similar sequence can be identified by the length of the extension products on gel electrophoresis. This simple procedure eliminates the much-demanding process of sequencing. We term this process Arrested Primer Extension (APE). As a demonstration of the feasibility of this method, we have used APE to speciate a known set of cultured mycobacteria. There should be many other applications of this method.

AB - This paper reports a novel method for the identification of nucleic acid target sequences when these targets have high sequence identity. Homologous genes are currently identified by sequencing. We hypothesize that by primer extension in the presence of selected nucleotides, genes with similar sequence can be identified by the length of the extension products on gel electrophoresis. This simple procedure eliminates the much-demanding process of sequencing. We term this process Arrested Primer Extension (APE). As a demonstration of the feasibility of this method, we have used APE to speciate a known set of cultured mycobacteria. There should be many other applications of this method.

UR - http://www.scopus.com/inward/record.url?scp=0027309473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027309473&partnerID=8YFLogxK

M3 - Article

C2 - 7687450

AN - SCOPUS:0027309473

VL - 14

SP - 951

EP - 955

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 6

ER -