Gene expression profile of murine long-term reconstituting vs. short-term reconstituting hematopoietic stem cells

Jiang F. Zhong, Yi Zhao, Susan Sutton, Andrew Su, Yuxia Zhan, Lunjian Zhu, Chunli Yan, Tim Gallaher, Patrick B. Johnston, W. French Anderson, Michael P. Cooke

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

The hematopoietic stem cell (HSC) compartment is composed of long-term reconstituting (LTR) and short-term reconstituting (STR) stem cells. LTR HSC can reconstitute the hematopoietic system for life, whereas STR HSC can sustain hematopoiesis for only a few weeks in the mouse. Several excellent gene expression profiles have been obtained of the total hematopoietic stem cell population. We have used five-color FACS sorting to isolate separate populations of LTR and STR stem cell subsets. The LTR HSC has the phenotype defined as Lin- Sca+ Kit+ 38+ 34-; two subsets of STR HSC were obtained with phenotypes of Lin- Sca + Kit+ 38+ 34+ and Lin- Sca+ Kit+ 38- 34+. The microarray profiling study reported here was able to identify genes specific for LTR functions. In the interrogated genes (≈12,000 probe sets corresponding to 8,000 genes), 210 genes are differentially expressed, and 72 genes are associated with LTR activity, including membrane proteins, signal transduction molecules, and transcription factors. Hierarchical clustering of the 210 differentially expressed genes suggested that they are not bone marrow-specific but rather appear to be stem cell-specific. Transcription factor-binding site analysis suggested that GATA3 might play an important role in the biology of LTR HSC.

Original languageEnglish (US)
Pages (from-to)2448-2453
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number7
DOIs
StatePublished - Feb 15 2005

Keywords

  • Microarray
  • Regulation

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Gene expression profile of murine long-term reconstituting vs. short-term reconstituting hematopoietic stem cells'. Together they form a unique fingerprint.

  • Cite this