Gene expression pattern associated with radiotherapy sensitivity in cervical cancer

Y. F. Wong, D. S. Sahota, T. H. Cheung, K. W K Lo, S. F. Yim, T. K H Chung, A. M Z Chang, David I Smith

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The objective of the present preliminary study was to determine if a difference in the pattern of gene expression exists between tumors that were subsequently found to be sensitive to radiotherapy and tumors found to be resistant to radiotherapy. PATIENTS AND METHODS: A total of 16 patients with invasive squamous cell carcinoma of the uterine cervix were included in this study. All patients were treated with standardized radiotherapy alone. Ten of the tumors were clinically radiosensitive and six were radioresistant. Total RNA, extracted from tumor specimens obtained prior to treatment, was hybridized onto an oligonucleotide microarray with probe sets complementary to over 20,000 transcripts. The genes were first subjected to a statistical filter to identify genes with statistically significant differential expression levels between those that were radiosensitive and those that were radioresistant. A back-propagation neural network was then constructed to model the differences so that patterns could be easily identified. RESULTS: Although a number of genes were found to express differentially between radiosensitive and radioresistant tumors; the 10 most discriminating genes were used to construct the model. Using the expressions from these 10 genes, we found that neural networks constructed from random subsets of the whole data were capable of predicting radiotherapy responses in the remaining subset, which appears stable within the dataset. DISCUSSION: This study shows that such an approach has the potential to differentiate tumor radiosensitivity, although confirmation of such a pattern using other larger independent datasets is necessary before firm conclusions can be drawn.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalCancer Journal
Volume12
Issue number3
DOIs
StatePublished - 2006

Fingerprint

Uterine Cervical Neoplasms
Radiotherapy
Gene Expression
Neoplasms
Genes
Radiation Tolerance
Oligonucleotide Array Sequence Analysis
Cervix Uteri
Squamous Cell Carcinoma
RNA
Datasets

Keywords

  • Cervical cancer
  • Gene expression
  • Microarray
  • Neural network
  • Radiosensitivity

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wong, Y. F., Sahota, D. S., Cheung, T. H., Lo, K. W. K., Yim, S. F., Chung, T. K. H., ... Smith, D. I. (2006). Gene expression pattern associated with radiotherapy sensitivity in cervical cancer. Cancer Journal, 12(3), 189-193. https://doi.org/10.1097/00130404-200605000-00006

Gene expression pattern associated with radiotherapy sensitivity in cervical cancer. / Wong, Y. F.; Sahota, D. S.; Cheung, T. H.; Lo, K. W K; Yim, S. F.; Chung, T. K H; Chang, A. M Z; Smith, David I.

In: Cancer Journal, Vol. 12, No. 3, 2006, p. 189-193.

Research output: Contribution to journalArticle

Wong, YF, Sahota, DS, Cheung, TH, Lo, KWK, Yim, SF, Chung, TKH, Chang, AMZ & Smith, DI 2006, 'Gene expression pattern associated with radiotherapy sensitivity in cervical cancer', Cancer Journal, vol. 12, no. 3, pp. 189-193. https://doi.org/10.1097/00130404-200605000-00006
Wong, Y. F. ; Sahota, D. S. ; Cheung, T. H. ; Lo, K. W K ; Yim, S. F. ; Chung, T. K H ; Chang, A. M Z ; Smith, David I. / Gene expression pattern associated with radiotherapy sensitivity in cervical cancer. In: Cancer Journal. 2006 ; Vol. 12, No. 3. pp. 189-193.
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