GDF-5 deficiency in mice delays Achilles tendon healing

A. Chhabra, D. Tsou, R. T. Clark, V. Gaschen, E. B. Hunziker, B. Mikic

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

The aim of this study was to examine the role of one of the growth/differentiation factors, GDF-5, in the process of tendon healing. Specifically, we tested the hypothesis that GDF-5 deficiency in mice would result in delayed Achilles tendon repair. Using histologic, biochemical, and ultrastructural analyses, we demonstrate that Achilles tendons from 8-week-old male GDF-5 -/- mice exhibit a short-term delay of 1-2 weeks in the healing process compared to phenotypically normal control littermates. Mutant animals took longer to achieve peak cell density, glycosaminoglycan content, and collagen content in the repair tissue, and the time course of changes in collagen fibril size was also delayed. Revascularization was delayed in the mutant mice by 1 week. GDF-5 deficient Achilles tendons also contained significantly more fat within the repair tissue at all time points examined, and was significantly weaker than control tissue at 5 weeks after surgery, but strength differences were no longer detectable by 12-weeks. Together, these data support the hypothesis that GDF-5 may play an important role in modulating tendon repair, and are consistent with previously posited roles for GDF-5 in cell recruitment, migration/adhesion, differentiation, proliferation, and angiogenesis.

Original languageEnglish (US)
Pages (from-to)826-835
Number of pages10
JournalJournal of Orthopaedic Research
Volume21
Issue number5
DOIs
StatePublished - Jan 1 2003

Keywords

  • Bone morphogenetic protiens
  • GDF-5
  • Growth/differentiation factors
  • Mouse
  • Tendon repair

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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  • Cite this

    Chhabra, A., Tsou, D., Clark, R. T., Gaschen, V., Hunziker, E. B., & Mikic, B. (2003). GDF-5 deficiency in mice delays Achilles tendon healing. Journal of Orthopaedic Research, 21(5), 826-835. https://doi.org/10.1016/S0736-0266(03)00049-4