Galectin-4 expression in carcinoid tumors

Kandelaria M. Rumilla, Lori A. Erickson, Alan K. Erickson, Ricardo V. Lloyd

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Galectins (Gal) are an evolutionarily conserved family of 15 carbohydrate-binding proteins (lectins) that are widely distributed in normal and neoplastic cells in a wide range of organisms. They have roles in inflammation, cell adhesion, tumor progression, and metastasis. The function and distribution of Gal-3 and Gal-1 are well characterized, but less information is available about Gal-4. Recent studies have localized Gal-4 in the enterochromaffin cells of the porcine and murine small intestine. We examined the expression of Gal-4 in primary and metastatic human ileal carcinoid tumors as well as in carcinoid tumors of the stomach, lung, and rectum. A total of 44 primary and 42 ileal metastatic carcinoid tumors were examined by immunohistochemistry using tissue microarrays (TMA) with monoclonal antibodies to Gal-4, Gal-3, and Gal-1. Pulmonary (n ≤ 7), rectal (n ≤ 6), and gastric (n ≤ 6) carcinoids were examined with larger tissue sections. A total of 18 pancreatic neuroendocrine tumors were also examined with larger tissue sections. Western blots of three ileal carcinoids were also done. Gal-4 was most highly expressed in the ileal carcinoids and the levels of expression tended to be higher in primary ileal carcinoids compared to the metastatic tumors (p ≤ 0.069). All 18 pancreatic neuroendocrine tumors were negative for Gal-1, Gal-3, and Gal-4. Western blot showed a 32 kDa band for Gal-4 in the ileal carcinoids. Gal-3 and Gal-1 were not detected in the metastatic ileal carcinoids by Western blotting. Gastric carcinoids also expressed Gal-4, but very few pulmonary or rectal carcinoids were positive for Gal-4 (p ≤ 0.002). Lower levels of Gal-1 and Gal-3 expression were present in ileal carcinoids compared to primary pulmonary and rectal tumors. These results show a differential distribution of Gal-4 in carcinoid tumors in different locations of the gastrointestinal tract and the lungs.

Original languageEnglish (US)
Pages (from-to)243-250
Number of pages8
JournalEndocrine Pathology
Volume17
Issue number3
DOIs
StatePublished - 2006

Fingerprint

Galectin 4
Carcinoid Tumor
Galectin 1
Galectin 3
Lung
Stomach
Neuroendocrine Tumors
Western Blotting
Enterochromaffin Cells
Galectins

Keywords

  • Carcinoid tumors
  • Galectin-3
  • Galectin-4
  • Galectins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Rumilla, K. M., Erickson, L. A., Erickson, A. K., & Lloyd, R. V. (2006). Galectin-4 expression in carcinoid tumors. Endocrine Pathology, 17(3), 243-250. https://doi.org/10.1385/EP:17:3:243

Galectin-4 expression in carcinoid tumors. / Rumilla, Kandelaria M.; Erickson, Lori A.; Erickson, Alan K.; Lloyd, Ricardo V.

In: Endocrine Pathology, Vol. 17, No. 3, 2006, p. 243-250.

Research output: Contribution to journalArticle

Rumilla, KM, Erickson, LA, Erickson, AK & Lloyd, RV 2006, 'Galectin-4 expression in carcinoid tumors', Endocrine Pathology, vol. 17, no. 3, pp. 243-250. https://doi.org/10.1385/EP:17:3:243
Rumilla KM, Erickson LA, Erickson AK, Lloyd RV. Galectin-4 expression in carcinoid tumors. Endocrine Pathology. 2006;17(3):243-250. https://doi.org/10.1385/EP:17:3:243
Rumilla, Kandelaria M. ; Erickson, Lori A. ; Erickson, Alan K. ; Lloyd, Ricardo V. / Galectin-4 expression in carcinoid tumors. In: Endocrine Pathology. 2006 ; Vol. 17, No. 3. pp. 243-250.
@article{ddd786b9caf14ce0a8e8a97f9d26ca24,
title = "Galectin-4 expression in carcinoid tumors",
abstract = "Galectins (Gal) are an evolutionarily conserved family of 15 carbohydrate-binding proteins (lectins) that are widely distributed in normal and neoplastic cells in a wide range of organisms. They have roles in inflammation, cell adhesion, tumor progression, and metastasis. The function and distribution of Gal-3 and Gal-1 are well characterized, but less information is available about Gal-4. Recent studies have localized Gal-4 in the enterochromaffin cells of the porcine and murine small intestine. We examined the expression of Gal-4 in primary and metastatic human ileal carcinoid tumors as well as in carcinoid tumors of the stomach, lung, and rectum. A total of 44 primary and 42 ileal metastatic carcinoid tumors were examined by immunohistochemistry using tissue microarrays (TMA) with monoclonal antibodies to Gal-4, Gal-3, and Gal-1. Pulmonary (n ≤ 7), rectal (n ≤ 6), and gastric (n ≤ 6) carcinoids were examined with larger tissue sections. A total of 18 pancreatic neuroendocrine tumors were also examined with larger tissue sections. Western blots of three ileal carcinoids were also done. Gal-4 was most highly expressed in the ileal carcinoids and the levels of expression tended to be higher in primary ileal carcinoids compared to the metastatic tumors (p ≤ 0.069). All 18 pancreatic neuroendocrine tumors were negative for Gal-1, Gal-3, and Gal-4. Western blot showed a 32 kDa band for Gal-4 in the ileal carcinoids. Gal-3 and Gal-1 were not detected in the metastatic ileal carcinoids by Western blotting. Gastric carcinoids also expressed Gal-4, but very few pulmonary or rectal carcinoids were positive for Gal-4 (p ≤ 0.002). Lower levels of Gal-1 and Gal-3 expression were present in ileal carcinoids compared to primary pulmonary and rectal tumors. These results show a differential distribution of Gal-4 in carcinoid tumors in different locations of the gastrointestinal tract and the lungs.",
keywords = "Carcinoid tumors, Galectin-3, Galectin-4, Galectins",
author = "Rumilla, {Kandelaria M.} and Erickson, {Lori A.} and Erickson, {Alan K.} and Lloyd, {Ricardo V.}",
year = "2006",
doi = "10.1385/EP:17:3:243",
language = "English (US)",
volume = "17",
pages = "243--250",
journal = "Endocrine Pathology",
issn = "1046-3976",
publisher = "Humana Press",
number = "3",

}

TY - JOUR

T1 - Galectin-4 expression in carcinoid tumors

AU - Rumilla, Kandelaria M.

AU - Erickson, Lori A.

AU - Erickson, Alan K.

AU - Lloyd, Ricardo V.

PY - 2006

Y1 - 2006

N2 - Galectins (Gal) are an evolutionarily conserved family of 15 carbohydrate-binding proteins (lectins) that are widely distributed in normal and neoplastic cells in a wide range of organisms. They have roles in inflammation, cell adhesion, tumor progression, and metastasis. The function and distribution of Gal-3 and Gal-1 are well characterized, but less information is available about Gal-4. Recent studies have localized Gal-4 in the enterochromaffin cells of the porcine and murine small intestine. We examined the expression of Gal-4 in primary and metastatic human ileal carcinoid tumors as well as in carcinoid tumors of the stomach, lung, and rectum. A total of 44 primary and 42 ileal metastatic carcinoid tumors were examined by immunohistochemistry using tissue microarrays (TMA) with monoclonal antibodies to Gal-4, Gal-3, and Gal-1. Pulmonary (n ≤ 7), rectal (n ≤ 6), and gastric (n ≤ 6) carcinoids were examined with larger tissue sections. A total of 18 pancreatic neuroendocrine tumors were also examined with larger tissue sections. Western blots of three ileal carcinoids were also done. Gal-4 was most highly expressed in the ileal carcinoids and the levels of expression tended to be higher in primary ileal carcinoids compared to the metastatic tumors (p ≤ 0.069). All 18 pancreatic neuroendocrine tumors were negative for Gal-1, Gal-3, and Gal-4. Western blot showed a 32 kDa band for Gal-4 in the ileal carcinoids. Gal-3 and Gal-1 were not detected in the metastatic ileal carcinoids by Western blotting. Gastric carcinoids also expressed Gal-4, but very few pulmonary or rectal carcinoids were positive for Gal-4 (p ≤ 0.002). Lower levels of Gal-1 and Gal-3 expression were present in ileal carcinoids compared to primary pulmonary and rectal tumors. These results show a differential distribution of Gal-4 in carcinoid tumors in different locations of the gastrointestinal tract and the lungs.

AB - Galectins (Gal) are an evolutionarily conserved family of 15 carbohydrate-binding proteins (lectins) that are widely distributed in normal and neoplastic cells in a wide range of organisms. They have roles in inflammation, cell adhesion, tumor progression, and metastasis. The function and distribution of Gal-3 and Gal-1 are well characterized, but less information is available about Gal-4. Recent studies have localized Gal-4 in the enterochromaffin cells of the porcine and murine small intestine. We examined the expression of Gal-4 in primary and metastatic human ileal carcinoid tumors as well as in carcinoid tumors of the stomach, lung, and rectum. A total of 44 primary and 42 ileal metastatic carcinoid tumors were examined by immunohistochemistry using tissue microarrays (TMA) with monoclonal antibodies to Gal-4, Gal-3, and Gal-1. Pulmonary (n ≤ 7), rectal (n ≤ 6), and gastric (n ≤ 6) carcinoids were examined with larger tissue sections. A total of 18 pancreatic neuroendocrine tumors were also examined with larger tissue sections. Western blots of three ileal carcinoids were also done. Gal-4 was most highly expressed in the ileal carcinoids and the levels of expression tended to be higher in primary ileal carcinoids compared to the metastatic tumors (p ≤ 0.069). All 18 pancreatic neuroendocrine tumors were negative for Gal-1, Gal-3, and Gal-4. Western blot showed a 32 kDa band for Gal-4 in the ileal carcinoids. Gal-3 and Gal-1 were not detected in the metastatic ileal carcinoids by Western blotting. Gastric carcinoids also expressed Gal-4, but very few pulmonary or rectal carcinoids were positive for Gal-4 (p ≤ 0.002). Lower levels of Gal-1 and Gal-3 expression were present in ileal carcinoids compared to primary pulmonary and rectal tumors. These results show a differential distribution of Gal-4 in carcinoid tumors in different locations of the gastrointestinal tract and the lungs.

KW - Carcinoid tumors

KW - Galectin-3

KW - Galectin-4

KW - Galectins

UR - http://www.scopus.com/inward/record.url?scp=33847136063&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847136063&partnerID=8YFLogxK

U2 - 10.1385/EP:17:3:243

DO - 10.1385/EP:17:3:243

M3 - Article

C2 - 17308361

AN - SCOPUS:33847136063

VL - 17

SP - 243

EP - 250

JO - Endocrine Pathology

JF - Endocrine Pathology

SN - 1046-3976

IS - 3

ER -