Gal repressosome contains an antiparallel DNA loop

Mark Geanacopoulos, George Vasmatzis, Victor B. Zhurkin, Sankar Adhya

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Gal repressosome assembly and repression of the galoperon in Escherichia coil occurs when two dimeric GalR proteins and the histone-like HU protein bind to cognate sites causing DNA looping. Structure-based genetic analysis defined the GalR surfaces interacting to form a stacked, V-shaped, tetrameric structure. Stereochemical models of the four possible DNA loops compatible with the GalR tetramer configuration were constructed using the sequence-dependent structural parameters of the interoperator DNA and conformation changes caused by GalR and asymmetric HU binding. Evaluation of their DNA elastic energies gave unambiguous preference to a loop structure in which the two gal operators adopt an antiparallel orientation causing undertwisting of DNA.

Original languageEnglish (US)
Pages (from-to)432-436
Number of pages5
JournalNature Structural Biology
Volume8
Issue number5
DOIs
StatePublished - 2001

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DNA
Nucleic Acid Conformation
Escherichia
Genetic Structures
Histones
Proteins
Conformations

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology

Cite this

Gal repressosome contains an antiparallel DNA loop. / Geanacopoulos, Mark; Vasmatzis, George; Zhurkin, Victor B.; Adhya, Sankar.

In: Nature Structural Biology, Vol. 8, No. 5, 2001, p. 432-436.

Research output: Contribution to journalArticle

Geanacopoulos, M, Vasmatzis, G, Zhurkin, VB & Adhya, S 2001, 'Gal repressosome contains an antiparallel DNA loop', Nature Structural Biology, vol. 8, no. 5, pp. 432-436. https://doi.org/10.1038/87595
Geanacopoulos, Mark ; Vasmatzis, George ; Zhurkin, Victor B. ; Adhya, Sankar. / Gal repressosome contains an antiparallel DNA loop. In: Nature Structural Biology. 2001 ; Vol. 8, No. 5. pp. 432-436.
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