GABAA receptor autoimmunity: A multicenter experience

Kevin O'Connor, Patrick Waters, Lars Komorowski, Anastasia Zekeridou, Chu Yueh Guo, Victor C. Mgbachi, Christian Probst, Swantje Mindorf, Bianca Teegen, Jeffrey M. Gelfand, Michael D. Geschwind, Vanda Lennon, Sean J. Pittock, Andrew McKeon

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

ObjectiveWe sought to validate methods for detection and confirmation of GABAA receptor (R)-IgG and clinically characterize seropositive cases.MethodsArchived serum and CSF specimens (185 total) suspected to harbor GABAAR-IgG were evaluated by indirect immunofluorescence assay (IFA). Twenty-six specimens from 19 patients appeared suspicious for GABAAR-IgG positivity by IFA, based on prior reports and comparison with commercial GABAAR antibody staining. Aliquots of those specimens were tested at the University of Oxford, United Kingdom, and Euroimmun, Lubeck, Germany, for GABAAR-IgG by cell-based assays (CBAs) using HEK293-indicator cells transfected with plasmids encoding different GABAAR subunits.ResultsEight specimens (of 26 tested; 4 serums, 4 CSFs) from 5 patients were confirmed by CBA to be GABAAR-IgG positive. Patient IgGs were always reactive with α1β3 GABAAR subunits. One more patient was identified clinically after this validation study. Median age for the 6 patients at serologic diagnosis was 44 years (range, 1-71 years), and 4 of them were male. Among the 4 for whom clinical information was available (2 treated by the authors), all had encephalitis and antiepileptic drug refractory seizures. Three out of 4 patients treated with a combination of immunotherapies had good outcomes. The fourth, recognized to have an autoimmune cause late in the clinical course, had severe permanent neurologic sequelae and brain atrophy.ConclusionsThough not as common as NMDA-R encephalitis, GABAAR encephalitis generally has a characteristic clinical-radiologic presentation and is treatable, making accurate laboratory diagnosis critical.

Original languageEnglish (US)
Article numbere552
JournalNeurology: Neuroimmunology and NeuroInflammation
Volume6
Issue number3
DOIs
StatePublished - May 1 2019

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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    O'Connor, K., Waters, P., Komorowski, L., Zekeridou, A., Guo, C. Y., Mgbachi, V. C., Probst, C., Mindorf, S., Teegen, B., Gelfand, J. M., Geschwind, M. D., Lennon, V., Pittock, S. J., & McKeon, A. (2019). GABAA receptor autoimmunity: A multicenter experience. Neurology: Neuroimmunology and NeuroInflammation, 6(3), [e552]. https://doi.org/10.1212/NXI.0000000000000552