Further evidence for a specific ganglioside fraction closely associated with myelin

Kunihiko Suzuki, Joseph F. Poduslo, Shirley E. Poduslo

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

1. 1. Myelin was isolated From gray and white matter separately, and naturally the yield of myelin from white matter was much higher. The white matter myelin from rat brain contains most of the gangliosides found in myelin from whole rat brain, even though the gray matter myelin contains twice as much ganglioside per unit weight as white matter myelin. 2. 2. Myelin isolated from either gray or white matter of adult bovine or rat brain had the characteristic myelin ganglioside pattern found previously for myelin prepared from adult rat whole brain; i.e. a predominance of the normal major monosialoganglioside G4 (GM1). 3. 3. Myelin was prepared from the mixed homogenate of unlabeled adult rat brains and neonatal rat brains previously injected with D-[1-14C]glucosamine. Only 0.2-0.3% of the neonatal brain ganglioside was recovered in the myelin, and more than 99.6% of G4 (GM1) in myelin derived from the adult brains. 4. 4. The fatty acid compositions of the major monosialoganglioside G4 (GM1) of whole gray matter, white matter and myelin were identical, with stearic acid comprising 90% of the total fatty acids. 5. 5. These findings show that the gangliosides in myelin from whole brain arise predominantly from white matter and are not due to random contamination by non-myelin tissue elements, particularly synaptic membranes. This study indicates the presence of a specific ganglioside fraction localized within the myelin sheath itself, or possibly in an axonal membrane closely associated with myelin.

Original languageEnglish (US)
Pages (from-to)576-586
Number of pages11
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume152
Issue number3
StatePublished - May 1 1968
Externally publishedYes

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Gangliosides
Myelin Sheath
Brain
Rats
Fatty Acids
Membranes
Glucosamine
Contamination
Synaptic Membranes
Tissue
White Matter

ASJC Scopus subject areas

  • Endocrinology
  • Biophysics
  • Biochemistry
  • Medicine(all)

Cite this

Further evidence for a specific ganglioside fraction closely associated with myelin. / Suzuki, Kunihiko; Poduslo, Joseph F.; Poduslo, Shirley E.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 152, No. 3, 01.05.1968, p. 576-586.

Research output: Contribution to journalArticle

Suzuki, Kunihiko ; Poduslo, Joseph F. ; Poduslo, Shirley E. / Further evidence for a specific ganglioside fraction closely associated with myelin. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1968 ; Vol. 152, No. 3. pp. 576-586.
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