Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure

Sarah M. Greising, Carlos Bernardo Mantilla, Gary C Sieck

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

The diaphragm muscle must be able to generate sufficient forces to accomplish a range of ventilatory and non-ventilatory behaviors throughout life. Measurements of transdiaphragmatic pressure (Pdi) can be conducted during eupnea, hypoxia (10 % O2)-hypercapnia (5 % CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths (i.e., sighs), sustained airway or tracheal occlusion, and maximal efforts elicited via bilateral phrenic nerve stimulation, representing the full range of motor behaviors available by the diaphragm muscle. We provide detailed methods on the in vivo measurements of Pdi in mice.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages309-319
Number of pages11
Volume1460
DOIs
StatePublished - Aug 6 2016

Publication series

NameMethods in Molecular Biology
Volume1460
ISSN (Print)10643745

Fingerprint

Respiratory Muscles
Diaphragm
Chemical Stimulation
Sneezing
Pressure
Muscles
Phrenic Nerve
Hypercapnia
Hypoxia

Keywords

  • Diaphragm muscle
  • Mouse
  • Muscle force
  • Non-ventilatory behavior
  • Phrenic nerve
  • Ventilatory behavior

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

Cite this

Greising, S. M., Mantilla, C. B., & Sieck, G. C. (2016). Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure. In Methods in Molecular Biology (Vol. 1460, pp. 309-319). (Methods in Molecular Biology; Vol. 1460). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3810-0_21

Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure. / Greising, Sarah M.; Mantilla, Carlos Bernardo; Sieck, Gary C.

Methods in Molecular Biology. Vol. 1460 Humana Press Inc., 2016. p. 309-319 (Methods in Molecular Biology; Vol. 1460).

Research output: Chapter in Book/Report/Conference proceedingChapter

Greising, SM, Mantilla, CB & Sieck, GC 2016, Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure. in Methods in Molecular Biology. vol. 1460, Methods in Molecular Biology, vol. 1460, Humana Press Inc., pp. 309-319. https://doi.org/10.1007/978-1-4939-3810-0_21
Greising SM, Mantilla CB, Sieck GC. Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure. In Methods in Molecular Biology. Vol. 1460. Humana Press Inc. 2016. p. 309-319. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3810-0_21
Greising, Sarah M. ; Mantilla, Carlos Bernardo ; Sieck, Gary C. / Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure. Methods in Molecular Biology. Vol. 1460 Humana Press Inc., 2016. pp. 309-319 (Methods in Molecular Biology).
@inbook{933bc971908040a98c79c5511b485879,
title = "Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure",
abstract = "The diaphragm muscle must be able to generate sufficient forces to accomplish a range of ventilatory and non-ventilatory behaviors throughout life. Measurements of transdiaphragmatic pressure (Pdi) can be conducted during eupnea, hypoxia (10 {\%} O2)-hypercapnia (5 {\%} CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths (i.e., sighs), sustained airway or tracheal occlusion, and maximal efforts elicited via bilateral phrenic nerve stimulation, representing the full range of motor behaviors available by the diaphragm muscle. We provide detailed methods on the in vivo measurements of Pdi in mice.",
keywords = "Diaphragm muscle, Mouse, Muscle force, Non-ventilatory behavior, Phrenic nerve, Ventilatory behavior",
author = "Greising, {Sarah M.} and Mantilla, {Carlos Bernardo} and Sieck, {Gary C}",
year = "2016",
month = "8",
day = "6",
doi = "10.1007/978-1-4939-3810-0_21",
language = "English (US)",
volume = "1460",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "309--319",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure

AU - Greising, Sarah M.

AU - Mantilla, Carlos Bernardo

AU - Sieck, Gary C

PY - 2016/8/6

Y1 - 2016/8/6

N2 - The diaphragm muscle must be able to generate sufficient forces to accomplish a range of ventilatory and non-ventilatory behaviors throughout life. Measurements of transdiaphragmatic pressure (Pdi) can be conducted during eupnea, hypoxia (10 % O2)-hypercapnia (5 % CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths (i.e., sighs), sustained airway or tracheal occlusion, and maximal efforts elicited via bilateral phrenic nerve stimulation, representing the full range of motor behaviors available by the diaphragm muscle. We provide detailed methods on the in vivo measurements of Pdi in mice.

AB - The diaphragm muscle must be able to generate sufficient forces to accomplish a range of ventilatory and non-ventilatory behaviors throughout life. Measurements of transdiaphragmatic pressure (Pdi) can be conducted during eupnea, hypoxia (10 % O2)-hypercapnia (5 % CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths (i.e., sighs), sustained airway or tracheal occlusion, and maximal efforts elicited via bilateral phrenic nerve stimulation, representing the full range of motor behaviors available by the diaphragm muscle. We provide detailed methods on the in vivo measurements of Pdi in mice.

KW - Diaphragm muscle

KW - Mouse

KW - Muscle force

KW - Non-ventilatory behavior

KW - Phrenic nerve

KW - Ventilatory behavior

UR - http://www.scopus.com/inward/record.url?scp=84981332603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84981332603&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3810-0_21

DO - 10.1007/978-1-4939-3810-0_21

M3 - Chapter

C2 - 27492181

AN - SCOPUS:84981332603

VL - 1460

T3 - Methods in Molecular Biology

SP - 309

EP - 319

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -