Abstract
The diaphragm muscle must be able to generate sufficient forces to accomplish a range of ventilatory and non-ventilatory behaviors throughout life. Measurements of transdiaphragmatic pressure (Pdi) can be conducted during eupnea, hypoxia (10 % O2)-hypercapnia (5 % CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths (i.e., sighs), sustained airway or tracheal occlusion, and maximal efforts elicited via bilateral phrenic nerve stimulation, representing the full range of motor behaviors available by the diaphragm muscle. We provide detailed methods on the in vivo measurements of Pdi in mice.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 309-319 |
| Number of pages | 11 |
| Volume | 1460 |
| DOIs | |
| State | Published - Aug 6 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1460 |
| ISSN (Print) | 10643745 |
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Keywords
- Diaphragm muscle
- Mouse
- Muscle force
- Non-ventilatory behavior
- Phrenic nerve
- Ventilatory behavior
ASJC Scopus subject areas
- Medicine(all)
- Molecular Biology
- Genetics
Cite this
Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure. / Greising, Sarah M.; Mantilla, Carlos Bernardo; Sieck, Gary C.
Methods in Molecular Biology. Vol. 1460 Humana Press Inc., 2016. p. 309-319 (Methods in Molecular Biology; Vol. 1460).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Functional measurement of respiratory muscle motor behaviors using transdiaphragmatic pressure
AU - Greising, Sarah M.
AU - Mantilla, Carlos Bernardo
AU - Sieck, Gary C
PY - 2016/8/6
Y1 - 2016/8/6
N2 - The diaphragm muscle must be able to generate sufficient forces to accomplish a range of ventilatory and non-ventilatory behaviors throughout life. Measurements of transdiaphragmatic pressure (Pdi) can be conducted during eupnea, hypoxia (10 % O2)-hypercapnia (5 % CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths (i.e., sighs), sustained airway or tracheal occlusion, and maximal efforts elicited via bilateral phrenic nerve stimulation, representing the full range of motor behaviors available by the diaphragm muscle. We provide detailed methods on the in vivo measurements of Pdi in mice.
AB - The diaphragm muscle must be able to generate sufficient forces to accomplish a range of ventilatory and non-ventilatory behaviors throughout life. Measurements of transdiaphragmatic pressure (Pdi) can be conducted during eupnea, hypoxia (10 % O2)-hypercapnia (5 % CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths (i.e., sighs), sustained airway or tracheal occlusion, and maximal efforts elicited via bilateral phrenic nerve stimulation, representing the full range of motor behaviors available by the diaphragm muscle. We provide detailed methods on the in vivo measurements of Pdi in mice.
KW - Diaphragm muscle
KW - Mouse
KW - Muscle force
KW - Non-ventilatory behavior
KW - Phrenic nerve
KW - Ventilatory behavior
UR - http://www.scopus.com/inward/record.url?scp=84981332603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84981332603&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3810-0_21
DO - 10.1007/978-1-4939-3810-0_21
M3 - Chapter
C2 - 27492181
AN - SCOPUS:84981332603
VL - 1460
T3 - Methods in Molecular Biology
SP - 309
EP - 319
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -